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Archives of Virology

, Volume 146, Issue 9, pp 1723–1738 | Cite as

Isolation and characterization of an endogenous cytomegalovirus (BaCMV) from baboons

  • E. L. Blewett
  • G. White
  • J. T. Saliki
  • R. Eberle

Summary.

 This report describes the isolation of CMV-like viruses from olive, yellow and chacma sub-species of baboons. The viruses were identified as CMVs by their characteristic growth properties in cell culture, virion morphology under the TEM, and antigenic cross-reactivity with other primate CMVs. The glycoprotein B gene homologue from an olive baboon CMV isolate (BaCMV OCOM4-37) was identified, cloned and sequenced. We present the sequence of this gene and by phylogenetic analysis demonstrate that BaCMV is in fact a cytomegalovirus, and is more closely related to rhesus CMV than to human CMV. An ELISA was developed to measure anti-BaCMV antibodies in baboon sera. Serological testing of colony-bred and wild-born baboons indicated that BaCMV is ubiquitous in all baboon populations, with > 95% of adult baboons of all sub-species being infected.

Keywords

Cell Culture Phylogenetic Analysis Serological Testing Characteristic Growth Gene Homologue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2001

Authors and Affiliations

  • E. L. Blewett
    • 1
  • G. White
    • 2
  • J. T. Saliki
    • 3
  • R. Eberle
    • 4
  1. 1. Department of Biochemistry and Microbiology, College of Osteopathic Medicine, Oklahoma State University, Tulsa, Oklahoma, U.S.A.US
  2. 2. Animal Resources, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma, U.S.A.US
  3. 3. Oklahoma Animal Disease Diagnostic Laboratory, Oklahoma State University, Stillwater, Oklahoma, U.S.A.US
  4. 4. Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma, U.S.A.US

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