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Archives of Virology

, Volume 145, Issue 7, pp 1359–1371 | Cite as

Genomic variability of equine herpesvirus-5

  • M. Dunowska
  • S. A. Holloway
  • C. R. Wilks
  • J. Meers

Summary.

 Seventeen New Zealand isolates of equine herpesvirus 5 (EHV-5) were compared to the Australian prototype strain. PCR primers were designed to amplify EHV-5 glycoprotein B (gB) gene, and Restriction Fragment Length Polymorphism (RFLP) was used to detect differences between cloned PCR products. EHV-5 isolates from different horses showed a high degree of heterogeneity. However, EHV-5 isolates from individual horses remained homogeneous when examined over a period of time or isolated from different sites. A single EHV-5 gB RFLP profile was detected in isolates from each individual horse but one. Two or possibly three different genotypes of EHV-5 were detected in cultures inoculated with a nasal swab of this horse. The heterogeneity observed between EHV-5 isolates from different horses suggests that the use of RFLP may provide a useful epidemiological approach to gain more knowledge about the biology of EHV-5.

Keywords

Fragment Length Length Polymorphism Restriction Fragment Length Polymorphism Restriction Fragment Fragment Length Polymorphism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2000

Authors and Affiliations

  • M. Dunowska
    • 1
  • S. A. Holloway
    • 2
  • C. R. Wilks
    • 1
  • J. Meers
    • 1
  1. 1. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New ZealandNZ
  2. 2. Centre for Equine Virology, The University of Melbourne, Parkville, AustraliaAU

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