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Archives of Virology

, Volume 144, Issue 8, pp 1619–1626 | Cite as

Nucleotide sequence of the 3′ terminal region of the genome of four Lettuce mosaic virus isolates from Greece and Yemen

  • F. Revers
  • R. A. A. van der Vlugt
  • S. Souche
  • M. Lanneau
  • H. Lot
  • T. Candresse
  • O. Le Gall
Brief Report

Summary

Lettuce mosaic virus (LMV) is an economically important Potyvirus causing a severe disease of commercial lettuce crops. Based on molecular data, three phylogenetic groups of isolates have previously been discriminated, reflecting their geographical origin (Western Europe-California, Greece, or Yemen). Sequence information for the entire coat protein domain was only available for one of the Western Europe-California phylogenetic group. We have now sequenced the 3′ terminal region of the genome LMV-Gr4, -Gr5 and -GrB, isolates which belong to the Greek phylogenetic group and of LMV-Yar, the sole known representative of the third LMV phylogenetic group. The region sequenced encodes the last 62 amino-acids of the polymerase and the entire coat protein of the four isolates, plus the 3′ non-translated region of LMV-Gr5 and -Yar. The Greek and Yemenite isolates studied are all very aggressive on lettuce, are able to overcome the resistance genes mo1 1 and mo1 2 and belong to the two phylogenetic groups which have so far been only partially characterised. As for other Potyviruses, the core and the C-terminal regions of the coat protein are highly conserved among all isolates whereas the N-terminus is more variable. No amino acid change in the coat protein or carboxy-terminal part of the polymerase could be related to the resistance-breaking properties of the isolates analysed. The sequences obtained provide the basis for the rapid typing of LMV isolates using the restriction pattern of segments of cDNA amplified by PCR.

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Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • F. Revers
    • 1
  • R. A. A. van der Vlugt
    • 2
  • S. Souche
    • 3
  • M. Lanneau
    • 1
  • H. Lot
    • 3
  • T. Candresse
    • 1
  • O. Le Gall
    • 1
  1. 1.Virologie Végétale, IBVM-INRA Bordeaux-Aquitaine, Villenave d’Ornon, FranceFrance
  2. 2.IPO-DLO, Wageningen, The NetherlandsThe Netherlands
  3. 3.Station de Pathologie Végétale, INRA, Montfavet, FranceFrance

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