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Archives of Virology

, Volume 142, Issue 11, pp 2249–2256 | Cite as

Identification of amino acids involved in a serotype and neutralization specific epitope with in the s1 subunit of avian infectious bronchitis virus

  • K. M. Moore
  • M. W. Jackwood
  • D. A. Hilt
Article

Summary

Localization of neutralizing, serotype specific epitopes of infectious bronchitis virus has been difficult because these epitopes are conformationally dependent. We identified amino acids involved in a serotype specific, conformationally dependent epitope by analysis of the S1 gene of 13 monoclonal antibody-neutralization-resistant mutants. Substitutions in the predicted amino acid sequence of these mutants were located at residues 304 and/or 386. Most of the substitutions at residue 304 were from threonine to isoleucine, whereas the substitutions at residue 386 were from arginine to proline, histidine, cysteine, or tryptophan. Based on this data, it appears that AA residues at 304 and 386 on the S1 glycoprotein are involved in a virus neutralizing serotype specific epitope.

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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • K. M. Moore
    • 1
  • M. W. Jackwood
    • 1
  • D. A. Hilt
    • 1
  1. 1.Department of Avian Medicine, University of Georgia, Athens, Georgia, U.S.A.USA

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