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Archives of Virology

, Volume 142, Issue 2, pp 255–270 | Cite as

Modified activity of a VP2-located neutralizing epitope on various vaccine, pathogenic and hypervirulent strains of infectious bursal disease virus

  • N. Eterradossi
  • D. Toquin
  • G. Rivallan
  • M. Guittet
Article

Summary

Nine monoclonal antibodies (Mabs) to a vaccine strain of infectious bursal disease virus (IBDV) of intermediate virulence were characterized in Western-blot, radioimmunoprecipitation, ELISA additivity, and neutralization assays. At least two distinct serotype 1-specific conformation-dependent overlapping neutralizing antigenic domains were shown to be present on IBDV-VP2, and were respectively probed by Mabs 3 and 4, and by Mabs 6 and 7. Ten serotype 1 vaccine or pathogenic IBDV strains were tested for neutralization. Most mild or intermediate vaccine strains were efficiently neutralized by all Mabs, whereas US variant A, European pathogenic strain Faragher 52/70 and French hypervirulent isolate 89 163 were not neutralized by Mabs 3 and 4. In addition, these two Mabs were shown to bind to the Faragher 52/70 strain, but not to the 89 163 isolate, in an antigen-capture ELISA. These results suggest that a neutralizing epitope is possibly modified in European pathogenic IBDV strains, and that Mabs 3 and 4 may prove useful for antigenic differentiation between European classical and hypervirulent isolates.

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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • N. Eterradossi
    • 1
  • D. Toquin
    • 1
  • G. Rivallan
    • 1
  • M. Guittet
    • 1
  1. 1.Centre National d’Etudes Vétérinaires et Alimentaires (CNEVA), Laboratoire Central de Recherches Avicole et Porcine (LCRAP), Unité de Recherches en Virologie, Immunologie et Parasitologie Aviaires et Cunicoles, Ploufragan, FranceFrance

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