Archives of Virology

, Volume 145, Issue 3, pp 523–534 | Cite as

Definition of an epitope on Japanese encephalitis virus (JEV) envelope protein recognized by JEV-specific murine CD8+ cytotoxic T lymphocytes

  • K. Takada
  • H. Masaki
  • E. Konishi
  • M. Takahashi
  • I. Kurane

Summary.

 We defined an epitope on the Japanese encephalitis virus (JEV) envelope (E) protein recognized by CD8+ cytotoxic T lymphocytes (CTLs). CTLs induced in JEV-infected BALB/c (H-2d) mice recognized E and/or premembrane (PrM) proteins, while CTLs in C57BL/6J (H-2b) and C3H/HeJ (H-2k) mice did not. JEV-specific CTLs had a phenotype of CD3+ CD4 CD8+. Twenty-four 9-amino acid (a.a.) peptides, which had binding motifs for H-2Kd, H-2Ld or H-2Dd, were synthesized according to the amino acid sequences of PrM and E proteins. CTLs induced by JEV infection recognized only the peptide K-3. Immunization of BALB/c mice with only a group of peptides including K-3 induced CTLs which recognized the homologous K-3 peptide, while immunization with other peptides did not. The peptide K-3 had a binding motif for H-2Kd. This is consistent with the finding that JEV-specific CTLs in BALB/c mice was H-2Kd-restricted.

These results indicate that the epitope recognized by CTLs in BALB/c mice is located between a.a. 60 and 68 on the E protein, corresponding to an a.a. sequence of CYHASVTDI.

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Copyright information

© 2000 Springer-Verlag/ Wien

Authors and Affiliations

  • K. Takada
    • 1
  • H. Masaki
    • 2
  • E. Konishi
    • 3
  • M. Takahashi
    • 1
  • I. Kurane
    • 2
  1. 1. Department of Neurology, Kinki University School of Medicine, Osakasayama, JapanJP
  2. 2. Department of Microbiology, Kinki University School of Medicine, Osakasayama, JapanJP
  3. 3. Department of Health Sciences, Kobe University School of Medicine, Kobe, JapanJP

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