Advertisement

Archives of Virology

, Volume 145, Issue 3, pp 473–489 | Cite as

Improvement of a serodiagnostic strategy for foot-and-mouth disease virus surveillance in cattle under systematic vaccination: a combined system of an indirect ELISA-3ABC with an enzyme-linked immunoelectrotransfer blot assay

  • I. E. Bergmann
  • V. Malirat
  • E. Neitzert
  • E. Beck
  • N. Panizzutti
  • C. Sánchez
  • A. Falczuk

Summary.

 Foot-and-mouth disease (FMD) recombinant non-capsideal viral antigens 3A, 3B, 2C, 3D and 3ABC were assessed individually in an indirect enzyme-linked immunosorbent assay (I-ELISA) for their ability to screen for persistent infection-specific antibodies in cattle, regardless of vaccination condition. Results of sequential serum samples from non-vaccinated animals with experimentally induced persistent infection, and their correlation with virus isolation, indicated that the polypeptides 3A, 3B and 3ABC showed the most adequate characteristics for further field studies.

Reliable performance of the I-ELISA with the selected antigen 3ABC was indicated by the distinct patterns observed for the frequency distribution values of naive and true positive samples. For regularly vaccinated livestock, a clear negative profile was proved in samples from regions without recent history of FMD. In contrast, at 90 and 900 days post-outbreak, coexistence of a positive and a negative population was established. These findings indicated that, irrespective of vaccination, the test allowed a classification of the herd-disease status.

A high degree of agreement was observed between I-ELISA-3ABC and EITB results for clearly reactive and non-reactive sera. For samples with reactivity values close to that of the cut-off, the EITB profiles upheld the definition of the infection condition. On this basis, screening by I-ELISA-3ABC, together with confirmation of suspect or positive samples by EITB is proposed as an adequate and accurate approach for large-scale epidemiological surveillance.

Keywords

Positive Sample Persistent Infection Vaccination Condition Epidemiological Surveillance Accurate Approach 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag/Wien 2000

Authors and Affiliations

  • I. E. Bergmann
    • 1
  • V. Malirat
    • 1
  • E. Neitzert
    • 1
  • E. Beck
    • 2
  • N. Panizzutti
    • 3
  • C. Sánchez
    • 1
  • A. Falczuk
    • 4
  1. 1. Pan-American Foot-and-Mouth Disease Center (PAHO/WHO), Rio de Janeiro, BrazilBR
  2. 2. Biochemisches Institut am Klinikum der Justus-Liebig Universität, Giessen, GermanyDE
  3. 3. Laboratório Regional de Apoio Animal-LARA/RS, Porto Alegre, BrazilBR
  4. 4. Gerencia de Laboratorios/Servicio Nacional de Sanidad Animal Gelab, SENASA, Buenos Aires, ArgentinaAR

Personalised recommendations