Abstract
Cases of acute haemorrhagic conjunctivitis (AHC) caused by a coxsackie virus A24 variant (CV-A24v) in Mexico have been reported since 1987; however, no molecular data on the causative strains have been available. Here, we report the identification of the etiological agent responsible for the most recent AHC outbreak in southeastern Mexico (at the end of 2017) as well as the complete genome sequences of seven isolates, using next-generation sequencing (NGS). Phylogenomic analysis of the CV-A24v sequences reported here showed similarity to contemporary strains causing AHC outbreaks in French Guiana and Uganda, forming a novel clade related to genotype IV. Moreover, a specific mutational pattern in the non-structural proteins was identified in the 2017 isolates. This is the first report of genetic characterization of CV-A24v isolates obtained in Mexico.
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References
Mirkovic RR, Kono R, Yin-Murphy M, Sohier R, Schmidt NJ, Melnick JL (1973) Enterovirus type 70: the etiologic agent of pandemic acute haemorrhagic conjunctivitis. Bull World Health Organ 49(4):341–346
Mirkovic RR, Schmidt NJ, Yin-Murphy M, Melnick JL (1974) Enterovirus etiology of the 1970 Singapore epidemic of acute conjunctivitis. Intervirology 4(2):119–127. https://doi.org/10.1159/000149850
Chang CH, Sheu MM, Lin KH, Chen CW (2001) Hemorrhagic viral keratoconjunctivitis in Taiwan caused by adenovirus types 19 and 37: applicability of polymerase chain reaction–restriction fragment length polymorphism in detecting adenovirus genotypes. Cornea 20(3):295–300. https://doi.org/10.1097/00003226-200104000-00011
Kono R (1975) Apollo 11 disease or acute hemorrhagic conjunctivitis: a pandemic of a new enterovirus infection of the eyes. Am J Epidemiol 101(5):383–390. https://doi.org/10.1093/oxfordjournals.aje.a112106
Ishiko H, Takeda N, Miyamura K, Kato N, Tanimura M, Lin KH, Yin-Murphy M, Tam JS, Mu GF, Yamazaki S (1992) Phylogenetic analysis of a coxsackievirus A24 variant: the most recent worldwide pandemic was caused by progenies of a virus prevalent around 1981. Virology 187(2):748–759. https://doi.org/10.1016/0042-6822(92)90477-7
Chu PY, Ke GM, Chang CH, Lin JC, Sun CY, Huang WL, Tsai YC, Ke LY, Lin KH (2009) Molecular epidemiology of coxsackie A type 24 variant in Taiwan, 2000–2007. J Clin Virol 45(4):285–291. https://doi.org/10.1016/j.jcv.2009.04.013
Yen YC, Chu PH, Lu PL, Lin YC, Shi YY, Chou LC, Wang CF, Lin YY, Su HJ, Lin CC, Zeng JY, Tyan YC, Ke GM, Chu PY (2016) Phylodynamic characterization of an ocular-tropism coxsackievirus A24 variant. PLoS One 11(8):e0160672. https://doi.org/10.1371/journal.pone.0160672
Centers for Disease Control (1987) Acute hemorrhagic conjunctivitis caused by coxsackievirus A24–Caribbean. MMWR Morb Mortal Wkly Rep 36:245–246
Tavares FN, de Campos RM, Burlandy FM, Fontella R, de Melo MM, da Costa EV, da Silva EE (2011) Molecular characterization and phylogenetic study of coxsackievirus A24v causing outbreaks of acute hemorrhagic conjunctivitis (AHC) in Brazil. PLoS One 6(8):89. https://doi.org/10.1371/journal.pone.0023206
Centers for Disease Control (1989) Acute hemorrhagic conjunctivitis–Mexico. MMWR Morb Mortal Wkly Rep 38(18):327–329
Dirección General de Epidemiología, Secretaría de Salud. Histórico Boletín Epidemiológico (Marzo de 1989, 2013 y semana epidemiológica 52 de 2018). https://www.gob.mx/salud/acciones-y-programas/historico-boletin-epidemiologico
Pan American Health Organization: Epidemiological Update: Conjunctivitis (July 25, 2017). https://www.paho.org/hq/dmdocuments/2017/2017-jul-25-phe-epi-update-conjunctivitis-virus.pdf
Piqueur MA, Verstrepen WA, Bruynseels P, Mertens AH (2009) Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA. Virol J 6:95. https://doi.org/10.1186/1743-422X-6-95
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular evolutionary genetics analysis version 60. Mol Biol Evol 30(12):2725–2729. https://doi.org/10.1093/molbev/mst197
Sousa IP Jr, Burlandy FM, Ferreira JL, Alves JCS, Sousa-Júnior EC, Tavares FN, da Silva EE (2019) Re-emergence of a coxsackievirus A24 variant causing acute hemorrhagic conjunctivitis in Brazil from 2017 to 2018. Arch Virol 164(4):1181–1185. https://doi.org/10.1007/s00705-019-04157-5
Laxmivandana R, Yergolkar P, Rajeshwari M, Chitambar SD (2014) Genomic characterization of coxsackievirus type A24 strains associated with acute flaccid paralysis and rarely identified Hopkins syndrome. Arch Virol 159(11):3125–3129. https://doi.org/10.1007/s00705-014-2129-9
Acknowledgements
The authors express their gratitude to Centro de Investigaciones Regionales “Dr. Hideyo Noguchi” and Laboratorios Estatales de Salud Pública (LESP) de Chiapas, Veracruz y Yucatán, for the clinical samples provided. N. E. E., J. E. R. G., and J. A. D. Q. received support from the Sistema Nacional de Investigadores (SNI) from CONACyT, México.
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Fragoso-Fonseca, D.E., Escobar-Escamilla, N., Rodríguez-Maldonado, A.P. et al. Complete genome sequence of a coxsackievirus type A24 variant causing an outbreak of acute haemorrhagic conjunctivitis in southeastern Mexico in 2017. Arch Virol 165, 1015–1018 (2020). https://doi.org/10.1007/s00705-020-04552-3
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DOI: https://doi.org/10.1007/s00705-020-04552-3