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Isolation and genomic characterization of a new mimivirus of lineage B from a Brazilian river

Abstract

Since its discovery, the first identified giant virus associated with amoebae, Acanthamoeba polyphaga mimivirus (APMV), has been rigorously studied to understand the structural and genomic complexity of this virus. In this work, we report the isolation and genomic characterization of a new mimivirus of lineage B, named “Borely moumouvirus”. This new virus exhibits a structure and replicative cycle similar to those of other members of the family Mimiviridae. The genome of the new isolate is a linear double-strand DNA molecule of ~1.0 Mb, containing over 900 open reading frames. Genome annotation highlighted different translation system components encoded in the DNA of Borely moumouvirus, including aminoacyl-tRNA synthetases, translation factors, and tRNA molecules, in a distribution similar to that in other lineage B mimiviruses. Pan-genome analysis indicated an increase in the genetic arsenal of this group of viruses, showing that the family Mimiviridae is still expanding. Furthermore, phylogenetic analysis has shown that Borely moumouvirus is closely related to moumouvirus australiensis. This is the first mimivirus lineage B isolated from Brazilian territory to be characterized. Further prospecting studies are necessary for us to better understand the diversity of these viruses so a better classification system can be established.

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Acknowledgements

We are grateful to our colleagues from Laboratório de Vírus of Universidade Federal de Minas Gerais. In addition, we thank CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), FAPEMIG (Fundação de Amparo à Pesquisa do estado de Minas Gerais), Ministério da Saúde (MS-DECIT) and the Microscopy Center of UFMG. J.S.A is a CNPq researcher. B.L.S. and J.S.A. are members of a CAPES-COFECUB project.

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Correspondence to Bernard La Scola or Jônatas Santos Abrahão.

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Dos Santos Silva, L.K., Rodrigues, R.A.L., dos Santos Pereira Andrade, A.C. et al. Isolation and genomic characterization of a new mimivirus of lineage B from a Brazilian river. Arch Virol (2020). https://doi.org/10.1007/s00705-020-04542-5

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