Advertisement

Novel positive-sense single-stranded RNA virus related to alphavirus-like viruses from Fusarium graminearum

  • Xing Zhang
  • Haotian Zhang
  • Dongfang Ma
  • Huaigu Chen
  • Wei LiEmail author
Annotated Sequence Record

Abstract

A putative novel positive-sense (+) RNA virus was detected in isolate CF16158 of the fungus Fusarium graminearum, the causal agent of Fusarium head blight and crown rot in wheat in China. The full genome of this virus was sequenced and characterized. The complete cDNA sequence is 7,051 nt long and contains four open reading frames (ORFs). ORF2 is predicted to encode helicase (Hel) and RNA-dependent RNA polymerase (RdRp) domains that are conserved among the alphavirus-like viruses. Pairwise comparisons and phylogenetic analysis of the deduced amino acid sequences of Hel and RdRp indicated that this (+) RNA mycovirus is a novel member of a new, yet to be established family of alphavirus-like viruses. Therefore, we named this virus “Fusarium graminearum alphavirus-like virus 1” (FgALV1). This is the first report of a full-length genomic sequence of a putative alphavirus-like virus in F. graminearum.

Notes

Acknowledgements

This work was supported by the National Key R&D Program of China (Grant no. 2018YFD0200500/2018YFD0200505) and the Special Fund for China Agricultural Research System (CARS-3).

Compliance with ethical standards

Conflict of interest

Xing Zhang, Haotian Zhang, Dongfang Ma, Huaigu Chen, and Wei Li declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Consent

All authors have seen and agree with the contents of the manuscript.

References

  1. 1.
    Chen X, He H, Yang X, Zeng H, Qiu D, Guo L (2016) The complete genome sequence of a novel Fusarium graminearum RNA virus in a new proposed family within the order Tymovirales. Arch Virol 161:2899–2903CrossRefGoogle Scholar
  2. 2.
    Chen Y, Kistler HC, Ma Z (2019) Fusarium graminearum trichothecene mycotoxins: biosynthesis, regulation, and management. Annu Rev Phytopathol 57:15–39CrossRefGoogle Scholar
  3. 3.
    Dean R, Van Kan JA, Pretorius ZA, Hammond-Kosack KE, Di Pietro A, Spanu PD, Rudd JJ, Dickman M, Kahmann R, Ellis J, Foster GD (2012) The Top 10 fungal pathogens in molecular plant pathology. Mol Plant Pathol 13:414–430CrossRefGoogle Scholar
  4. 4.
    Dweba CC, Figlan S, Shimelis HA, Motaung TE, Sydenham S, Mwadzingeni L, Tsilo TJ (2017) Fusarium head blight of wheat: pathogenesis and control strategies. Crop Prot 91:114–122CrossRefGoogle Scholar
  5. 5.
    Ghabrial SA, Castón JR, Jiang DH, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368CrossRefGoogle Scholar
  6. 6.
    Gilbert K, Holcomb EE, Allscheid RL, Carrington J (2019) Discovery of new mycoviral genomes within publicly available fungal transcriptomic datasets. BioRxiv 510404Google Scholar
  7. 7.
    Katoh K, Kuma K, Toh H, Miyata T (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Res 33:511–518CrossRefGoogle Scholar
  8. 8.
    Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Mentjies P, Drummond A (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28(12):1647–1649CrossRefGoogle Scholar
  9. 9.
    Koonin EV, Dolja VV (1993) Evolution and taxonomy of positive strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28:375–430CrossRefGoogle Scholar
  10. 10.
    Koonin EV, Dolja VV, Krupovic M (2015) Origins and evolution of viruses of eukaryotes: the ultimate modularity. Virology 479:2–25CrossRefGoogle Scholar
  11. 11.
    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  12. 12.
    Li P, Bhattacharjee P, Wang S, Zhang L, Ahmed I, Guo L (2019) Mycoviruses in Fusarium species: an update. Front Cell Infect Microbiol 9:257CrossRefGoogle Scholar
  13. 13.
    Liu H, Fu Y, Jiang D, Li G, Xie J, Peng Y, Yi X, Ghabrial SA (2009) A novel mycovirus that is related to the human pathogen hepatitis E virus and rubi-like viruses. J Virol 83(4):1981–1991CrossRefGoogle Scholar
  14. 14.
    Nicholson P, Simpson DR, Weston G, Rezanoor HN, Lees AK, Parry DW (1998) Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiol Mol Plant Pathol 53:17–37CrossRefGoogle Scholar
  15. 15.
    Picarelli MAS, Forgia M, Rivas EB, Nerva L, Chiapello M, Turina M, Colariccio A (2019) Extreme diversity of mycoviruses present in isolates of Rhizoctonia solani AG2-2 LP from Zoysia japonica from Brazil. Front Cell Infect Microbiol 9:244CrossRefGoogle Scholar
  16. 16.
    Starr EP, Nuccio EE, Pett-Ridge J, Banfield JF, Firestone MK (2019) Metatranscriptomic reconstruction reveals RNA viruses with the potential to shape carbon cycling in soil. bioRxiv 597468Google Scholar
  17. 17.
    Wolf YI, Kazlauskas D, Iranzo J, Lucía-Sanz A, Kuhn JH, Krupovic M, Dolja VV, Koonin EV (2018) Origins and evolution of the global RNA virome. mBio 9:e02329–e02418CrossRefGoogle Scholar
  18. 18.
    Xie J, Jiang D (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68CrossRefGoogle Scholar
  19. 19.
    Zhang XX, Sun HY, Shen CM, Li W, Yu HS, Chen HG (2015) Survey of Fusarium spp. causing wheat crown rot in major winter wheat growing regions of China. Plant Dis 99:1610–1615CrossRefGoogle Scholar
  20. 20.
    Zhu JZ, Zhu HJ, Gao BD, Zhou Q, Zhong J (2018) Diverse, novel mycoviruses from the virome of a hypovirulent Sclerotium rolfsii strain. Front Plant Sci 9:1738CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Hubei Collaborative Innovation Center for Grain IndustryYangtze UniversityJingzhouChina
  2. 2.Institute of Plant ProtectionJiangsu Academy of Agricultural SciencesNanjingChina
  3. 3.Jiangsu Co-Innovation Center for Modern Production Technology of Grain CropsYangzhou UniversityYangzhouChina

Personalised recommendations