Genomic characterization of Malus domestica virus A (MdoVA), a novel velarivirus infecting apple

  • Igor KoloniukEmail author
  • Jaroslava Přibylová
  • Jana Fránová
  • Josef Špak
Annotated Sequence Record


Screening of apple samples using a high-throughput sequencing (HTS) approach led to the discovery of a novel virus, tentatively named “Malus domestica virus A” (MdoVA). Its genomic organisation and phylogenetic relationship showed relatedness to viruses of the genus Velarivirus in the family Closteroviridae. It is not clear whether MdoVA has any impact on its host, as the analysed apple tree contained other viruses and a viroid.



We kindly thank Alena Matyášová for her technical assistance. This research was supported by the project QK1910065 of the Czech Ministry of Agriculture and by the institutional support RVO60077344.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

705_2019_4478_MOESM1_ESM.xlsx (12 kb)
Supplementary material 1 (XLSX 12 kb)
705_2019_4478_MOESM2_ESM.xlsx (11 kb)
Supplementary material 2 (XLSX 11 kb)


  1. 1.
    Hadidi A, Barba M (2018) Chapter 1: economic impact of pome and stone fruit viruses and viroids. In: Virus and virus-like diseases of pome and stone fruits. The American Phytopathological Society, pp 1–7Google Scholar
  2. 2.
    James D, Varga A, Jesperson GD et al (2013) Identification and complete genome analysis of a virus variant or putative new foveavirus associated with apple green crinkle disease. Arch Virol 158:1877–1887. CrossRefPubMedGoogle Scholar
  3. 3.
    Yaegashi H, Yoshikawa N, Candresse T (2018) Chapter 4: apple chlorotic leaf spot virus in pome fruits. In: Virus and virus-like diseases of pome and stone fruits. The American Phytopathological Society, pp 17–21Google Scholar
  4. 4.
    Petrzik K, Lenz O (2018) Chapter 6: Apple mosaic virusin pome fruits. In: Virus and virus-like diseases of pome and stone fruits. The American Phytopathological Society, pp 25–28Google Scholar
  5. 5.
    Camacho C, Coulouris G, Avagyan V et al (2009) BLAST+: architecture and applications. BMC Bioinform 10:421. CrossRefGoogle Scholar
  6. 6.
    Martelli GP, Agranovsky AA, Bar-Joseph M et al (2012) Family—Closteroviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier, San Diego, pp 987–1001Google Scholar
  7. 7.
    Dereeper A, Guignon V, Blanc G et al (2008) robust phylogenetic analysis for the non-specialist. Nucleic Acids Res 36:W465–W469. CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Letunic I, Bork P (2016) Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res 44:W242–W245. CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Rubio L, Guerri J, Moreno P (2013) Genetic variability and evolutionary dynamics of viruses of the family Closteroviridae. Front Microbiol 4:1–15. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Plant Virology, Institute of Plant Molecular Biology, Biology CentreCzech Academy of SciencesCeske BudejoviceCzech Republic

Personalised recommendations