Comparative thermostability analysis of zoonotic and human influenza virus A and B neuraminidase

  • Vasily A. EvseenkoEmail author
  • Svetlana V. Svyatchenko
  • Natalia P. Kolosova
  • Valentina L. Kovrizhkina
  • Vasiliy Y. Marchenko
  • Aleksander G. Durymanov
  • Natalia I. Goncharova
  • Alexander B. Ryzhikov
Brief Report


Neuraminidase (NA) thermostability of influenza A and B viruses isolated from birds, swine and humans was measured to evaluate its variability associated with host body temperature. The highest 50% inactivation temperature (IT50) was observed with H3N8 avian influenza virus (74 °C), and the lowest IT50 was observed with the seasonal human H3N2 virus (45.5 °C). The IT50 values of A(H1N1)pdm09 viruses 56.4-58.5 °C were statistically higher than that of the prepandemic strain A/Solomon Islands/03/06 (52.5 °C). An analysis of Ca2+ binding sites revealed the correspondence of amino acid changes to NA thermostability. This study demonstrates that changes in NA thermostability correspond to differences in host body temperature.



The authors thank the CDC (GA, USA), WHO CC for Reference and Research on Influenza, Australia, and Crick Worldwide Influenza Centre, UK, for providing virus strains through IRR and for sharing sequences through GISAID. The authors are especially grateful to the SRC VB “Vector” core sequence facilities.


This research was funded by the Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor).

Compliance with ethical standards

Conflict of interest

Evseenko V.A. declares that he has no conflict of interest. Kolosova N.P. declares that she has no conflict of interest. Svyatchenko S.V. declares that she has no conflict of interest. Kovrizhkina V.L. declares that she has no conflict of interest. Durymanov A.G. declares that he has no conflict of interest. Goncharova N.A. declares that she has no conflict of interest. Marchenko V.Y. declares that he has no conflict of interest. Ryzhikov A.B. declares that he has no conflict of interest.

Ethical approval

All applicable international, national and institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Vasily A. Evseenko
    • 1
    Email author
  • Svetlana V. Svyatchenko
    • 1
  • Natalia P. Kolosova
    • 1
  • Valentina L. Kovrizhkina
    • 1
  • Vasiliy Y. Marchenko
    • 1
  • Aleksander G. Durymanov
    • 1
  • Natalia I. Goncharova
    • 1
  • Alexander B. Ryzhikov
    • 1
  1. 1.State Research Center of Virology and Biotechnology “Vector”, RospotrebnadzorNovosibirsk RegionRussian Federation

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