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First characterization of a canine parvovirus causing fatal disease in coatis (Nasua nasua)

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Abstract

A canine parvovirus (CPV)-like virus was detected by PCR and isolated from dead coatis in Argentina. Analysis of the full-length genome sequence revealed that it resembled CPV-but also contained a mutation in the VP2 protein (Arg377Ser) that has not been described previously. This is the first report of a CPV-like virus producing clinical disease in coatis. Genetic similarity to CPV-2c viruses detected in Brazil suggests a strong relationship between these viruses. Although the pathogenic potential of CPV- and feline panleukopenia virus (FPV)-like strains in wild animals is still not completely understood, this study highlights the importance of parvoviruses as a threat to wildlife if proper conditions are present.

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Funding

This research did not receive any specific grant from funding agencies in the public, commercial or not-for-profit sectors.

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Authors and Affiliations

  1. Cátedra de Virología, Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Av. Chorroarín 280, C1427CWO, Ciudad Autónoma de Buenos Aires, Argentina

    Danilo Bucafusco, Leandro Diaz & Ana Bratanich

  2. Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Argentina

    Danilo Bucafusco, Hernán Argibay & Celina Vega

  3. Instituto de Ecología, Genética y Evolución de Buenos Aires, Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Ciudad Autónoma de Buenos Aires, Argentina

    Hernán Argibay

  4. Instituto de Virología, Instituto Nacional de Tecnología Agropecuaria, Hurlingham, Buenos Aires, Argentina

    Celina Vega

  5. Cátedra de Patología Básica, Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Ciudad Autónoma de Buenos Aires, Argentina

    Leonardo Minatel & Gabriela C. Postma

  6. Parque Ecológico “El Puma”, Ministerio de Ecología, Candelaria, Misiones, Argentina

    Miguel Rinas

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  1. Danilo Bucafusco
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Correspondence to Danilo Bucafusco.

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Supplementary Fig. 1

(A) Jejunum. Necrosis and dilatation of crypts of Lieberkühn, with villous atrophy (H&E, 100X) (left). Dilated crypts with cellular debris in the lumen. Some crypts are lined by flattened epithelial cells (H&E, 400X) (right). (B) MDCK and CRFK cells infected with the coati parvovirus and CPV-2c. FICT-labeled monoclonal antibody against CPV was used for specific antigen detection. Cellular nuclei were stained with DAPI (400X) (TIFF 4186 kb)

Supplementary Fig. 2

PCR for detection of carnivore protoparvovirus 1 DNA in supernatants from MDCK and CRFK after the 3rd passage of infection. (1) Infected MDCK, (2) mock MDCK, (3) infected CRFK, (4) Mock CRFK, (5) positive control, (6) negative control (7) 100-bp DNA ladder (TIFF 50 kb)

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Bucafusco, D., Argibay, H., Diaz, L. et al. First characterization of a canine parvovirus causing fatal disease in coatis (Nasua nasua). Arch Virol 164, 3073–3079 (2019). https://doi.org/10.1007/s00705-019-04417-4

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