The major oligosaccharyl transferase complex genes are not involved in dengue virus replication in Aedes aegypti mosquitoes
Replication of the dengue virus (DENV) genome occurs in a vesicle in the endoplasmic reticulum by a complex of host and viral proteins. Two host proteins, STT3A and STT3B, as members of the oligosaccharyl transferase complex, have been implicated in playing structural roles in the vesicle in mammalian cells, and the absence of these proteins has been shown to decrease DENV replication. Aedes aegypti is the main vector of the virus and has been used previously as a model organism to study mosquito-virus interactions. In this study, we found that genes of the oligosaccharyl transferase complex have no effect on replication of DENV in mosquito cells.
The authors would like to thank Dr. Daniel Watterson and Professor Paul Young from the University of Queensland for providing anti-DENV-2 antibodies used for virus titration. This project was supported by an Australian Research Council (DP150101782) to SA, and a University of Queensland scholarship to HDP.