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The major oligosaccharyl transferase complex genes are not involved in dengue virus replication in Aedes aegypti mosquitoes

  • Hugo D. Perdomo
  • Sassan AsgariEmail author
Brief Report

Abstract

Replication of the dengue virus (DENV) genome occurs in a vesicle in the endoplasmic reticulum by a complex of host and viral proteins. Two host proteins, STT3A and STT3B, as members of the oligosaccharyl transferase complex, have been implicated in playing structural roles in the vesicle in mammalian cells, and the absence of these proteins has been shown to decrease DENV replication. Aedes aegypti is the main vector of the virus and has been used previously as a model organism to study mosquito-virus interactions. In this study, we found that genes of the oligosaccharyl transferase complex have no effect on replication of DENV in mosquito cells.

Notes

Acknowledgements

The authors would like to thank Dr. Daniel Watterson and Professor Paul Young from the University of Queensland for providing anti-DENV-2 antibodies used for virus titration. This project was supported by an Australian Research Council (DP150101782) to SA, and a University of Queensland scholarship to HDP.

Supplementary material

705_2019_4376_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 12 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Australian Infectious Disease Research Centre, School of Biological SciencesThe University of QueenslandBrisbaneAustralia

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