In-depth proteomic profiling of the Singapore grouper iridovirus virion
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Singapore grouper iridovirus (SGIV) is a lethal grouper virus containing 162 predicted ORFs. Previous proteomic studies led to identification of 73 SGIV structural proteins. Here, SDS-assisted tube-gel digestion and DOC-assisted in-solution digestion coupled with LC-ESI-MS/MS were applied to further profile the SGIV structural proteome. We identified a total of 90 SGIV structural proteins including 24 newly reported proteins. Additionally, several PTMs were identified, including 26 N-terminal acetylated proteins, three phosphorylated proteins, and one myristoylated protein. Importantly, 47 of the proteins that were identified are predicted to contain conserved domains. Our work greatly expands the repertoire of the SGIV structural proteome and provides more insight into the biology of SGIV.
relative and absolute quantification
tandem mass spectrometry
matrix-assisted laser desorption ionization
open reading frame
sodium dodecyl sulfate
one-dimensional gel electrophoresis
We acknowledge Dr. Jinlu Wu of the National University of Singapore for kindly providing us with grouper embryonic cells and SGIV. This work was sponsored by the National Natural Science Foundation of China (no. 31702387) and the China Postdoctoral Science Foundation (no. 2018M630973).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The research reported here did not involve experimentation with human participants or animals.
- 4.Teng Y, Hou ZW, Gong J, Liu H, Xie XY, Zhang L, Chen XH, Qin QW (2008) Whole-genome transcriptional profiles of a novel marine fish iridovirus, Singapore grouper iridovirus (SGIV) in virus-infected grouper spleen cell cultures and in orange-spotted grouper, Epinephelus coioides. Virology 377(1):39–48CrossRefGoogle Scholar
- 14.Wang JG, Lee YM, Li CX, Li P, Li Z, Lim TK, Gong ZY, Lin QS (2015) Dramatic improvement of proteomic analysis of zebrafish liver tumor by effective protein extraction with sodium deoxycholate and heat denaturation. Int J Anal Chem 2015:1–11Google Scholar