Advertisement

Characterization of potato virus Y populations in potato in Israel

  • Liat Avrahami-Moyal
  • Yehudit Tam
  • Noa Sela
  • Shanmugam Prakash
  • Yael Meller Harel
  • Menachem Bornstein
  • Ran Shulchani
  • Zion Dar
  • Victor GabaEmail author
Brief Report
  • 44 Downloads

Abstract

Potato virus Y (PVY) is the most common virus infecting potato worldwide. We analysed potato tuber PVY infections from the major Israeli growing region in 2014-2017. Isolates were characterized by multiplex PCR according to Chikh-Ali et al. (Plant Disease 97, 1370, 2013), whose primers were not fully compatible with the Israeli isolates. New primers were designed for a multiplex PCR assay to differentiate the Israeli isolates. Three recombinant strains were observed: PVYNTNa (72% of the isolates), PVYNWi (24%) and PVYSyr-III (found only in 2015). The archetypal PVYO strain was found only once. The classical PVY strains have recently been displaced by recombinant forms, with PVYNTNa dominating. The Israeli isolates appear very similar to those of Europe (the seed tuber source), except for PVYSyr-III.

Notes

Acknowledgments

We acknowledge the contribution of the Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel, no. 591/19. The authors thank the Chief Scientist’s Office of the Israeli Ministry of Agricultural and Rural Development (grant no. 132171614) for support.

Authors’ contributions

Liat Avrahami-Moyal: designed and carried out virology and sequencing, analyzed results, wrote the manuscript. Yehudit Tam: virology, ELISA identification of infected plants. Noa Sela: bioinformatics. Shanmugam Prakash: virology and sequencing. Yael Meller Harel: virology and sequencing. Menachem Bornstein and Ran Shulchani: assisted in experiments, care and maintenance of plants and growth facilities. Zion Dar: co-designed the project, advised on agricultural matters. Victor Gaba: initiated and designed the project, analyzed data, wrote the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare there are no conflicts of interest.

Research involving human participants and/or animals

The research did not involve human participants or animals.

Informed consent

The research did not involve human participants or animals.

Supplementary material

705_2019_4250_MOESM1_ESM.docx (1.1 mb)
Supplementary material 1 (DOCX 1150 kb)
705_2019_4250_MOESM2_ESM.docx (19 kb)
Supplementary material 2 (DOCX 19 kb)

References

  1. 1.
    Anfoka G, Ahmad FH, Altaleb M, Al Shhab M, Abubaker S, Levy D, Rosner A, Czosnek H (2014) First report of recombinant Potato virus Y strains infecting potato in Jordan. Plant Dis 98:1017Google Scholar
  2. 2.
    Avrahami-Moyal L, Tam Y, Brumin M, Prakash S, Leibman D, Pearlsman M, Bornstein M, Sela N, Zeidan M, Dar Z, Zig U, Gaba V (2017) Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR. Phytoparasitica 45:591–598CrossRefGoogle Scholar
  3. 3.
    Bai Y, Han S, Gao Y, Zhang W, Fan G, Qiu C, Nie X, Wen J (2019) Genetic diversity of Potato virus Y (PVY) in potato production areas in Northeast China. Plant Dis 103:289–297CrossRefGoogle Scholar
  4. 4.
    Benedict CA, McMoran DW, Inglis DA, Karasev AV (2015) Tuber symptoms associated with recombinant strains of Potato virus Y in specialty potatoes under Western Washington growing conditions. Am J Pot Res 92:593–602CrossRefGoogle Scholar
  5. 5.
    Carroll JE, Smith DM, Gray SM (2016) Preferential acquisition and inoculation of PVYNTN over PVYO in potato by the green peach aphid Myzus persicae (Sulzer). J Gen Vir 97:797–802CrossRefGoogle Scholar
  6. 6.
    Chikh-Ali M, Alruwaili H, Vander Pol D, Karasev AV (2016) Molecular characterization of recombinant strains of Potato virus Y from Saudi Arabia. Plant Dis 100:292–297CrossRefGoogle Scholar
  7. 7.
    Chikh-Ali M, Bosque-Pérez NA, Vander Pol D, Sembel D, Karasev AV (2016) Occurrence and molecular characterization of recombinant Potato virus YNTN isolates from Sulawesi, Indonesia. Plant Dis 100:269–275CrossRefGoogle Scholar
  8. 8.
    Chikh-Ali M, Gray SM, Karasev AV (2013) An improved multiplex IC-RT-PCR assay distinguishes nine strains of Potato virus Y. Plant Dis 97:1370–1374CrossRefGoogle Scholar
  9. 9.
    Chikh Ali M, Maoka T, Natsuaki T, Natsuaki KT (2010) PVYNTN-NW, a novel recombinant strain of Potato virus Y predominating in potato fields in Syria. Plant Path 59:31–41CrossRefGoogle Scholar
  10. 10.
    Chikh-Ali M, Rodriguez-Rodriguez M, Green K, Kim DJ, Chung SM, Kuhl J, Karasev AV (2019) Identification and molecular characterization of recombinant Potato virus Y (PVY) in potato from South Korea, PVYNTN strain. Plant Dis 103:137–142CrossRefGoogle Scholar
  11. 11.
    Davie K, Holmes R, Pickup J, Lacomme C (2017) Dynamics of PVY strains in field grown potato: Impact of strain competition and ability to overcome host resistance mechanisms. Virus Res 241:95–104CrossRefGoogle Scholar
  12. 12.
    Elwan EA, Abdel Aleem EE, Fattouh FA, Green KJ, Tran LT, Karasev AV (2017) Occurrence of diverse recombinant strains of Potato virus Y circulating in potato fields in Egypt. Plant Dis 101:1463–1469CrossRefGoogle Scholar
  13. 13.
    Funke CN, Nikolaeva OV, Green KJ, Tran LT, Chikh-Ali M, Quintero-Ferrer A, Cating RA, Frost KE, Hamm PB, Olsen N, Pavek MJ (2017) Strain-specific resistance to Potato virus Y (PVY) in potato and its effect on the relative abundance of PVY strains in commercial potato fields. Plant Dis 101:20–28CrossRefGoogle Scholar
  14. 14.
    Glais L, Bellstedt DU, Lacomme C (2017) Diversity, characterisation and classification of PVY. In: Lacomme C et al (eds) Potato virus Y: biodiversity, pathogenicity, epidemiology and management. Springer, Cham, pp 43–76CrossRefGoogle Scholar
  15. 15.
    Green KJ, Brown CJ, Gray SM, Karasev AV (2017) Phylogenetic study of recombinant strains of Potato virus Y. Virol 507:40–52CrossRefGoogle Scholar
  16. 16.
    Green KJ, Brown CJ, Karasev AV (2018) Genetic diversity of potato virus Y (PVY): sequence analyses reveal ten novel PVY recombinant structures. Arch Virol 163:23–32CrossRefGoogle Scholar
  17. 17.
    Karasev AV, Gray SM (2013) Continuous and emerging challenges of Potato virus Y in potato. Annu Rev Phytopathol 51:571–586CrossRefGoogle Scholar
  18. 18.
    Luria N, Smith E, Reingold V, Bekelman I, Lapidot M, Levin I, Elad N, Tam Y, Sela N, Abu-Ras A, Ezra N (2017) A new Israeli tobamovirus isolate infects tomato plants harboring Tm-22 resistance genes. PloS one 12:p.e0170429CrossRefGoogle Scholar
  19. 19.
    Loebenstein G, Gaba V (2011) Viruses of potato. Adv Virus Res 84:209–246CrossRefGoogle Scholar
  20. 20.
    MacKenzie TD, Lavoie J, Nie X, Singh M (2018) Differential spread of Potato virus Y (PVY) strains O, N: O and NTN in the field: implications for the rise of recombinant PVY Strains in New Brunswick, Canada. Am J Potato Res 95:301–310CrossRefGoogle Scholar
  21. 21.
    Marco S (1981) Incidence of potato virus Y and potato leaf roll virus in autumn potatoes in Israel. Potato Res 24:353–356CrossRefGoogle Scholar
  22. 22.
    Schubert J, Fomitcheva V, Sztangret-Wiśniewska J (2007) Differentiation of Potato virus Y strains using improved sets of diagnostic PCR-primers. J Virol Met 140:66–74CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Liat Avrahami-Moyal
    • 1
  • Yehudit Tam
    • 1
  • Noa Sela
    • 1
  • Shanmugam Prakash
    • 1
  • Yael Meller Harel
    • 1
    • 2
  • Menachem Bornstein
    • 1
  • Ran Shulchani
    • 1
  • Zion Dar
    • 3
  • Victor Gaba
    • 1
    Email author
  1. 1.Department of Plant Pathology and Weed ScienceAgricultural Research Organization—The Volcani CenterRishon LeZionIsrael
  2. 2.Division for Plant Pests and Diseases, Plant Protection and Inspection ServicesMinistry of Agriculture and Rural DevelopmentBet DaganIsrael
  3. 3.Extension ServiceMinistry of Agriculture and Rural DevelopmentBet DaganIsrael

Personalised recommendations