A novel picornavirus in feces of a rainbow lorikeet (Trichoglossus moluccanus) shows a close relationship to members of the genus Avihepatovirus

  • Haoning Wang
  • Shixing Yang
  • Tongling ShanEmail author
  • Xiaolong Wang
  • Xutao Deng
  • Eric Delwart
  • Wen ZhangEmail author
Annotated Sequence Record


A novel picornavirus, named “lorikeet picornavirus 1” (LoPV-1), was detected in a fecal sample from rainbow lorikeets using viral metagenomic analysis, and its complete genome sequence was determined and analyzed. The genome of LoPV-1 is 7862 nt long, including a 617-nt 5’ UTR, a type IV IRES 5’UTR with an ‘8-like’ motif, a 7032-nt polyprotein ORF, and a 213-nt 3’ UTR. Phylogenetic analysis and pairwise asequence comparisons based on the amino acid sequences of P1, P2, and P3 indicated that LoPV-1 showed the closest relationship to two picornaviruses that were isolated recently from red-crowned cranes and clustered together with members of the genus Avihepatovirus.



This work was partly supported by the National Key Research and Development Programs of China (no. 2017YFC1200201), Jiangsu Provincial Key Research and Development Projects (no. BE2017693), and the National Natural Science Foundation (no. 31872478).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study did not include experiments with human participants or animals performed by any of the authors. No animals were harmed during sample collection.

Informed consent

Not applicable.

Supplementary material

705_2019_4246_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 16 kb)


  1. 1.
    King AMQ, Lefkowitz EJ, Mushegian AR et al (2018) Changes to taxonomy and the international code of virus classification and nomenclature ratified by the International Committee on Taxonomy of Viruses (2018). Arch Virol 163:2601–2631. CrossRefGoogle Scholar
  2. 2.
    Zell R, Delwart E, Gorbalenya AE et al (2017) ICTV virus taxonomy profile: picornaviridae. J Gen Virol 98:2421–2422. CrossRefGoogle Scholar
  3. 3.
    Zell R (2018) Picornaviridae-the ever-growing virus family. Arch Virol 163:299–317. CrossRefGoogle Scholar
  4. 4.
    Yugo DM, Hauck R, Shivaprasad HL, Meng X-J (2016) Hepatitis virus infections in poultry. Avian Dis 60:576–588. CrossRefGoogle Scholar
  5. 5.
    Shi S, Chen H, Chen Z et al (2013) Complete genome sequence of a duck hepatitis a virus 1 isolated from a pigeon in china. Genome Announc. Google Scholar
  6. 6.
    Wang Y, Yang S, Liu D et al (2018) The fecal virome of red-crowned cranes. Arch Virol. Google Scholar
  7. 7.
    Zhang W, Yang S, Shan T et al (2017) Virome comparisons in wild-diseased and healthy captive giant pandas. Microbiome 5:90. CrossRefGoogle Scholar
  8. 8.
    Pinto FL, Lindblad P (2010) A guide for in-house design of template-switch-based 5′ rapid amplification of cDNA ends systems. Anal Biochem 397:227–232. CrossRefGoogle Scholar
  9. 9.
    Tseng C-H, Tsai H-J (2007) Molecular characterization of a new serotype of duck hepatitis virus. Virus Res 126:19–31. CrossRefGoogle Scholar
  10. 10.
    Gao J, Chen J, Si X et al (2012) Genetic variation of the VP1 gene of the virulent duck hepatitis A virus type 1 (DHAV-1) isolates in Shandong province of China. Virol Sin 27:248–253. CrossRefGoogle Scholar
  11. 11.
    Liu G, Wang F, Ni Z et al (2008) Genetic diversity of the VP1 gene of duck hepatitis virus type I (DHV-I) isolates from southeast China is related to isolate attenuation. Virus Res 137:137–141. CrossRefGoogle Scholar
  12. 12.
    Wang A, Gu L, Wu S, Zhu S (2018) Duck hepatitis A virus structural proteins expressed in insect cells self-assemble into virus-like particles with strong immunogenicity in ducklings. Vet Microbiol 215:23–28. CrossRefGoogle Scholar
  13. 13.
    Asnani M, Kumar P, Hellen CUT (2015) Widespread distribution and structural diversity of Type IV IRESs in members of Picornaviridae. Virology 478:61–74. CrossRefGoogle Scholar
  14. 14.
    Wang H, Zhang W, Yang S et al (2018) Asian black bear (Ursus thibetanus) picornavirus related to seal aquamavirus A. Arch Virol. Google Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Haoning Wang
    • 1
    • 2
  • Shixing Yang
    • 3
  • Tongling Shan
    • 2
    Email author
  • Xiaolong Wang
    • 1
  • Xutao Deng
    • 4
  • Eric Delwart
    • 4
  • Wen Zhang
    • 3
    Email author
  1. 1.College of Wildlife ResourceNortheast Forestry UniversityHarbinChina
  2. 2.Department of Swine Infectious DiseaseShanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesShanghaiChina
  3. 3.Department of Microbiology, School of MedicineJiangsu UniversityZhenjiangChina
  4. 4.Vitalant Research InstituteSan FranciscoUSA

Personalised recommendations