Genomic sequence of a Bohle iridovirus strain isolated from a diseased boreal toad (Anaxyrus boreas boreas) in a North American aquarium

  • Kuttichantran Subramaniam
  • Thomas B. Waltzek
  • V. Gregory ChincharEmail author
Annotated Sequence Record


Genomic sequence analysis of zoo ranavirus (ZRV) suggests it is a strain of Bohle iridovirus (BIV), a virus that was first detected in, and thought to be confined to, Australia. Furthermore, marked sequence similarity and genomic co-linearity among ZRV, BIV, and German gecko ranavirus (GGRV) are consistent with the view that all three are strains  of Frog virus 3, the type species of the genus Ranavirus, family Iridoviridae.



We thank Mr. Kamonchai Imnoi for his technical assistance throughout the study.


No funding was received.

Compliance with ethical standards

Conflict of interest

All authors declare that there is no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.

Supplementary material

705_2019_4244_MOESM1_ESM.pdf (116 kb)
Fig. S1 Determination of sequence identity. Genetic analysis of the concatenated nucleotide (nt) sequences of 26 core iridovirus genes was performed using the BioEdit Sequence Alignment Editor v7.2.5, and the percent sequence identity among the isolates was determined. See Table S1 for virus abbreviations (PDF 117 kb)
705_2019_4244_MOESM2_ESM.docx (13 kb)
Supplementary material 2 (DOCX 13 kb)


  1. 1.
    Speare R, Smith JR (1992) An iridovirus-like agent isolated from the ornate burrowing frog Limnodynastes ornatus in northern Australia. Dis Aquat Organ 14:51–57CrossRefGoogle Scholar
  2. 2.
    Hick PM, Subramaniam K, Thompson P, Whittington RJ, Waltzek TB (2016) Complete genome sequence of a Bohle iridovirus isolate from ornate burrowing frogs (Limnodynastes ornatus) in Australia. Genome Announc 4:e00632-16CrossRefGoogle Scholar
  3. 3.
    Marschang RE, Braun S, Becher P (2005) Isolation of a ranavirus from a gecko (Uroplatus fimbriatus). J Zoo Wildl Med 36:295–300CrossRefGoogle Scholar
  4. 4.
    Stohr AC, Lopez-Bueno A, Blahak S, Caeiro M, Rosa G, Alves de Matos A, Martel A, Alejo A, Marschang R (2015) Phylogeny and differentiation of reptilian and amphibian ranaviruses detected in Europe. PLoS One 10(2):e0118633CrossRefGoogle Scholar
  5. 5.
    Une Y, Sakuma A, Matsueda H, Nakai K, Murakami M (2009) Ranavirus outbreak in North American bullfrogs (Rana catesbeiana), Japan, 2008. Emerg Infect Dis 15:1146–1147CrossRefGoogle Scholar
  6. 6.
    Picco AM, Collins JP (2008) Amphibian commerce as a likely source of pathogen pollution. Conserv Biol 22:1582–1589CrossRefGoogle Scholar
  7. 7.
    Cunningham AA, Daszak P, Wood JLN (2017) One Health, emerging infectious diseases and wildlife: two decades of progress? Philos Trans R Soc Lond B Biol Sci 372:20160167CrossRefGoogle Scholar
  8. 8.
    Cheng K, Jones ME, Jancovich JK, Burchell J, Schrenzel MD, Reavill DR, Imai DM, Urban A, Kirkendall M, Woods LW, Chinchar VG, Pessier AP (2014) Isolation of a Bohle-like iridovirus from boreal toads housed within a cosmopolitan aquarium collection. Dis Aquat Organ 111:139–152CrossRefGoogle Scholar
  9. 9.
    Ariel E, Subramaniam K, Imnoi K, Sriwanayos P, Ahasan MS, Olesen NJ, Amedeo M, Toffan A, Waltzek TB (2017) Genomic sequencing of ranaviruses isolated from edible frogs (Pelophylax esculentus). Genome Announc 5:e01015–e01017CrossRefGoogle Scholar
  10. 10.
    Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477CrossRefGoogle Scholar
  11. 11.
    Tcherepanov V, Ehlers A, Upton C (2006) Genome annotation transfer utility (GATU): rapid annotation of viral genomes using a closely related reference genome. BMC Genomics 7:150CrossRefGoogle Scholar
  12. 12.
    Tan WG, Barkman TJ, Chinchar VG, Essani K (2004) Comparative genomic analyses of frog virus 3, type species of the genus Ranavirus (family Iridoviridae). Virology 323:70–84CrossRefGoogle Scholar
  13. 13.
    Eaton HE, Metcalf J, Penny E, Tcherepanov V, Upton C, Brunetti CR (2007) Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes. Virol J 4:11CrossRefGoogle Scholar
  14. 14.
    Jancovich JK, Bremont M, Touchman JW, Jacobs BL (2010) Evidence for multiple recent host species shifts among the Ranaviruses (family Iridoviridae). J Virol 84:2636–2647CrossRefGoogle Scholar
  15. 15.
    Brodie R, Roper RL, Upton C (2004) JDotter: a Java interface to multiple dotplots generated by dotter. Bioinformatics 20:279–281CrossRefGoogle Scholar
  16. 16.
    Chen G, Ward BM, Yu KH, Chinchar VG, Robert J (2011) Improved knockout methodology reveals that frog virus 3 mutants lacking either the 18K immediate-early gene or the truncated vIF-2 alpha gene are defective for replication and growth in vivo. J Virol 85:11131–11138CrossRefGoogle Scholar
  17. 17.
    Rothenburg S, Chinchar VG, Dever TE (2011) Characterization of a ranavirus inhibitor of the antiviral protein kinase PKR. BMC Microbiol 11:56CrossRefGoogle Scholar
  18. 18.
    Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Infectious Diseases and Immunology, College of Veterinary MedicineUniversity of FloridaGainesvilleUSA
  2. 2.Department of Microbiology and ImmunologyUniversity of Mississippi Medical CenterJacksonUSA

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