Occurrence of tomato leaf curl Bangladesh virus and associated subviral DNA molecules in papaya in Bangladesh: molecular detection and characterization

  • Islam Hamim
  • Wayne B. Borth
  • Michael J. Melzer
  • Jon Y. Suzuki
  • Marisa M. Wall
  • John S. HuEmail author
Brief Report


Forty-five papaya samples showing severe leaf curl symptoms were tested by PCR with a degenerate primer set for virus species in the genus Begomovirus. Of these, 29 were positive for tomato leaf curl Bangladesh virus (ToLCBV). The complete genome sequences of ToLCBV (GenBank accession no. MH380003) and its associated tomato leaf curl betasatellite (ToLCB) (MH397223) from papaya isolate Gaz17-Pap were determined and characterized. Defective betasatellites were found in ToLCBV-positive papaya isolates Gaz19-Pap, Gaz20-Pap and Gaz21-Pap. This study confirmed that papaya is a host of ToLCBV, ToLCB, and other defective and recombinant DNA satellites in Bangladesh.



This work was supported by the United States Agency for International Development (Feed the Future initiative–CGIAR fund- BFS-G-1100002 and the predecessor fund of the Food Security and Crisis Mitigation II grant- EEM-G-0004-00013), the USDA National Institute of Food and Agriculture, Hatch HAW09025-H (1001478) and the USDA-Agricultural Research Service (58-5320-4-012).

Compliance with ethical standards

Conflict of interest

The authors declare there are no conflicts of interest.

Ethical approval

This article does not contain any studies with human or animal subjects performed by the any of the authors.

Supplementary material

705_2019_4235_MOESM1_ESM.doc (4.3 mb)
Supplementary material 1 (DOC 4354 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Plant and Environmental Protection SciencesUniversity of Hawaii at ManoaHonoluluUSA
  2. 2.Department of Plant PathologyBangladesh Agricultural UniversityMymensinghBangladesh
  3. 3.USDA-ARS, Daniel K. Inouye, U.S. Pacific Basin Agricultural Research CenterHiloUSA

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