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Archives of Virology

, Volume 164, Issue 8, pp 2187–2191 | Cite as

Detection and genome characterization of a novel member of the genus Polerovirus from zucchini (Cucurbita pepo) in China

  • Bin Peng
  • Baoshan Kang
  • Huijie Wu
  • Lifeng Liu
  • Liming Liu
  • Zhangjun Fei
  • Ni Hong
  • Qinsheng GuEmail author
Annotated Sequence Record
  • 83 Downloads

Abstract

Here, we report a novel member of the genus Polerovirus, zucchini aphid-borne yellows virus (ZABYV), which was identified in zucchini grown for seed production in the Xinjiang Uygur Autonomous Region, China. The complete nucleotide sequence of the ZABYV genome was determined and found to be 5,792 nucleotides in length, and like those of other poleroviruses, to contain seven open reading frames (ORFs). Multiple sequence alignments and phylogenetic analysis indicated that ZABYV is a new member of the genus Polerovirus, although several regions of its genome are closely related to chickpea chlorotic stunt virus (CpCSV). Further comparative analysis suggested that ZABYV originated from a recombination event between CpCSV and another unknown virus in the genus Polerovirus.

Notes

Acknowledgements

We would like to thank Dr. Xuejun Zhang for assistance with the collection of zucchini samples, and Yang Wu and Suyun Qiao for technical assistance. This work was supported by Modern Agro-Industry Technology Research System (CARS-26-13), the Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2015-ZFRI) and the Basic and Advanced Technology Program of Henan Province (152300410138).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2019_4217_MOESM1_ESM.docx (26 kb)
Supplementary material 1 (DOCX 25 kb)
705_2019_4217_MOESM2_ESM.docx (24 kb)
Supplementary material 2 (DOCX 24 kb)

References

  1. 1.
    Ibaba JD, Laing MD, Gubba A (2016) Pepo aphid-borne yellows virus: a new species in the genus Polerovirus. Virus Genes 53:1–3Google Scholar
  2. 2.
    King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2011) Virus taxonomy: Ninth report of the international committee on taxonomy of viruse. Elsevier Academic, AmsterdamGoogle Scholar
  3. 3.
    Knierim D, Deng TC, Tsai WS, Green SK, Kenyon L (2010) Molecular identification of three distinct Polerovirus species and a recombinant Cucurbit aphid-borne yellows virus strain infecting cucurbit crops in Taiwan. Plant Pathol 59:991–1002CrossRefGoogle Scholar
  4. 4.
    Knierim D, Maiss E, Kenyon L, Winter S, Menzel W (2015) First full-length genome sequence of the polerovirus luffa aphid-borne yellows virus (LABYV) reveals the presence of at least two consensus sequences in an isolate from Thailand. Arch Virol 160:2633–2636CrossRefGoogle Scholar
  5. 5.
    Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549CrossRefPubMedCentralGoogle Scholar
  6. 6.
    Martin DP, Murrell B, Golden M, Khoosal A, Muhire B (2015) RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol 1:1–5CrossRefGoogle Scholar
  7. 7.
    Montero-Pau J, Blanca J, Bombarely A, Ziarsolo P, Esteras C, Martí-Gómez C, Ferriol M, Gómez P, Jamilena M, Mueller L (2018) De novo assembly of the zucchini genome reveals a whole-genome duplication associated with the origin of the Cucurbita genus. Plant Biotechnol J 16:1161–1171CrossRefGoogle Scholar
  8. 8.
    Peng B, Liu LM, Liu SS, Zhang YH, Ji YL, Wu Y, Zhang XJ, Gu QS (2017) The genome of Cucurbit chlorotic yellows virus from Xinjiang autonomous region of China. Acta Phytopathol Sin 47:730–737Google Scholar
  9. 9.
    Ren C, Hao X, Du Y, Xiang B (2016) Molecular detection of seed zucchini virus disease in Shihezi and Xinhu region in Xinjiang. Northern Hort 40:102–106Google Scholar
  10. 10.
    Shang QX, Xiang HY, Han CG, Li DW, Yu JL (2009) Distribution and molecular diversity of three cucurbit-infecting poleroviruses in China. Virus Res 145:341–346CrossRefGoogle Scholar
  11. 11.
    Wu Y, Li J, Peng B, Gu Q (2017) Detection of virus infecting watermelon and melon in Henan, Gansu provinces and Xinjiang Autonomous Region. China Veg 8:31–35Google Scholar
  12. 12.
    Xiang HY, Shang QX, Han CG, Li DW, Yu JL (2008) Complete sequence analysis reveals two distinct poleroviruses infecting cucurbits in China. Arch Virol 153:1155–1160CrossRefGoogle Scholar
  13. 13.
    Zheng Y, Gao S, Padmanabhan C, Li R, Galvez M, Gutierrez D, Fuentes S, Ling KS, Kreuze J, Fei Z (2017) VirusDetect: an automated pipeline for efficient virus discovery using deep sequencing of small RNAs. Virology 500:130–138CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Henan Provincial Key Laboratory of Fruit and Cucurbit Biology, Zhengzhou Fruit Research InstituteChinese Academy of Agricultural ScienceZhengzhouPeople’s Republic of China
  2. 2.Boyce Thompson InstituteIthacaUSA
  3. 3.United States Department of Agriculture-Agricultural Research ServiceRobert W. Holley Center for Agriculture and HealthIthacaUSA
  4. 4.Huazhong Agricultural UniversityWuhanPeople’s Republic of China

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