Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus
- 39 Downloads
We describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-derived cells were cubic (0.1-2 μm) and localized in both the nucleus and cytoplasm, whereas those formed in Spodoptera frugiperda-derived cells were ovate and ellipsoidal (0.1-3 μm) and also localized in both the nucleus and cytoplasm. The molecular basis for differences in the morphology, size, and location of cypovirus occlusion bodies is unclear, and cellular proteins might play a role in their formation and location.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant number 407908/2013-7) and Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF, grant number 193.001532/2016).
- 2.Coulibaly F, Chiu E, Gutmann S, Rajendran C, Haebel PW, Ikeda K, Mori H, Ward VK, Schulze-Briese C, Metcalf P (2009) The atomic structure of baculovirus polyhedra reveals the independent emergence of infectious crystals in DNA and RNA viruses. Proc Natl Acad Sci USA 106:22205–22210. https://doi.org/10.1073/pnas.0910686106 CrossRefGoogle Scholar
- 5.Ginn HM, Messerschmidt M, Ji X, Zhang H, Axford D, Gildea RJ, Winter G, Brewster AS, Hattne J, Wagner A, Grimes JM, Evans G, Sauter NK, Sutton G, Stuart DI (2015) Structure of CPV17 polyhedrin determined by the improved analysis of serial femtosecond crystallographic data. Nat Commun 6:6435. https://doi.org/10.1038/ncomms7435 CrossRefGoogle Scholar
- 7.Horta AB, Ardisson-Araujo DMP, da Silva LA, de Melo FL, da Silva Morgado F, Franco Lemos MV, Ribeiro ZA, Boiça AL, Wilcken CF, Ribeiro BM (2018) Genomic analysis of a cypovirus isolated from the eucalyptus brown looper, Thyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae). Virus Res 253:62–67. https://doi.org/10.1016/j.virusres.2018.05.026 CrossRefGoogle Scholar
- 13.Mori H, Ito R, Nakazawa H, Sumida M, Matsubara F, Minobe Y (1993) Expression of Bombyx mori cytoplasmic polyhedrosis virus polyhedrin in insect cells by using a baculovirus expression vector, and its assembly into polyhedra. J Gen Virol 74(1):99–102. https://doi.org/10.1099/0022-1317-74-1-99 CrossRefGoogle Scholar
- 14.O’Reilly DR, Miller LK, Luckow VA (1992) Baculovirus expression vector: a laboratory manual. WH Freeman and Company, New YorkGoogle Scholar
- 16.Rohrmann G, By E (2013) Baculovirus molecular biology: introduction to the baculoviruses, their taxonomy, and evolution. National Center for Biotechnology Information, BethesdaGoogle Scholar