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First detection and genotypic analysis of goat enzootic nasal tumor virus 2 in Chongqing, China

  • Chao Ye
  • Qingyuan Huang
  • Tingting Chen
  • Jiali Jiang
  • Fengqing Hou
  • Dongyi Xu
  • Yuanyi Peng
  • Rendong FangEmail author
  • Jing ChenEmail author
Brief Report

Abstract

Enzootic nasal adenocarcinoma (ENA) of goats, characterized by transformation of epithelial cells of the ethmoid turbinates, is caused by enzootic nasal tumor virus 2 (ENTV-2). ENTV-2 belongs to the genus Betaretrovirus and has extended its distribution globally with a high prevalence; however, the genetic diversity and genotypic distribution for ENTV-2 have not been analyzed systematically due to the limited availability of sequence data. In this study, an infection by ENTV-2 was detected by RT-PCR in Chongqing in July 2018, and the complete sequence of one strain (CQ1) was determined. Phylogenetic analysis indicated a high degree of genetic heterogeneity among ENTV-2 sequences, with the existence of two main lineages. Lineage 1 and 2 were composed of ENTV-2 from China and the UK, respectively. Although CQ1 was closely related to recent ENTV-2 strains collected in the neighboring provinces of Chongqing (Shaanxi and Sichuan), it formed a separate sublineage of lineage 1 (sublineage 1.3). This report will enhance our understanding of the epidemiology of ENTV-2 in China.

Keywords:

Enzootic nasal tumor virus 2 Virus detection Chongqing Genotypic analysis Lineages 

Notes

Funding

This work was supported by the National Key Research and Development Program of China (2018YFD0500500), the Chongqing Science & Technology Commission (cstc2018jscx-msybX0302, cstc2018jscx-msybX0221), the earmarked fund for China Agriculture Research System (CARS-37), the Fundamental Research Funds for the Central Universities (XDJK2017A003, SWU118072), and the Innovation Team Building Program in Chongqing Universities (CXTDG201602004).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

705_2019_4211_MOESM1_ESM.docx (116 kb)
Supplementary material 1 (DOCX 115 kb)
705_2019_4211_MOESM2_ESM.jpg (380 kb)
Fig. S1 Pairwise comparisons between CQ1 and other strains based on the whole genome sequence (A) and an alignment of sequences of representative ENTV-2 strains based on the ORFx amino acid sequence (B). (JPEG 379 kb)
705_2019_4211_MOESM3_ESM.docx (15 kb)
Supplementary material 3 (DOCX 14 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Chao Ye
    • 1
  • Qingyuan Huang
    • 1
  • Tingting Chen
    • 1
  • Jiali Jiang
    • 1
  • Fengqing Hou
    • 1
  • Dongyi Xu
    • 1
  • Yuanyi Peng
    • 1
  • Rendong Fang
    • 1
    Email author
  • Jing Chen
    • 2
    Email author
  1. 1.College of Animal Science and TechnologySouthwest UniversityChongqingChina
  2. 2.Chongqing Academy of Animal SciencesChongqingChina

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