Archives of Virology

, Volume 164, Issue 5, pp 1459–1467 | Cite as

Establishment of a rescue system for porcine parvovirus using a seamless cloning method

  • Lingling Zhang
  • Dou Gao
  • Yongle Yu
  • Yebing Liu
  • Weiquan Liu
  • Jinxiang Li
  • Shangjin CuiEmail author
Brief Report


In this study, we describe a novel and rapid method for the construction of a full-length infectious clone (pPPV). The constructed clone contained an engineered EcoRv site that served as a genetic marker and was shown to be infectious when transfected into a monolayer of PK-15 cells. The rescued virus (rPPV) of the infectious clone was found to be indistinguishable from wild-type virus BQ in terms of its biological properties. The generation of this PPV infectious clone provides a potentially powerful tool with which to elucidate the molecular pathogenesis of PPV.


Porcine parvovirus Infectious clone Genetic marker Rescued virus Biological properties 



This work was supported by the Agricultural Science and Technology Innovation Program (ASTIP-IAS15), National Natural Science Foundation of China (NO. 31172349 & NO. 31172341) and the National Key Research and Development Program of China (No. 2016YFD0501003 & 2017YFD0502300).

Compliance with ethical standards

Conflicts of interest

All authors declare they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants and the animals studies was approved by the Science Research Department (in charge of animal welfare issue) of the Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (IAS-CAAS) (Beijing, China).

Informed consent

The author unanimously agreed to submit the manuscript to Archives of Virology.


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Lingling Zhang
    • 1
    • 2
  • Dou Gao
    • 1
    • 2
  • Yongle Yu
    • 3
  • Yebing Liu
    • 4
  • Weiquan Liu
    • 3
  • Jinxiang Li
    • 5
  • Shangjin Cui
    • 1
    • 2
    Email author
  1. 1.Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Technology of Beijing, Ministry of AgricultureBeijingChina
  3. 3.State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological SciencesChina Agricultural UniversityBeijingChina
  4. 4.China Institute of Veterinary Drugs ControlBeijingChina
  5. 5.Chinese Academy of Agricultural SciencesBeijingChina

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