Establishment of a rescue system for porcine parvovirus using a seamless cloning method
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In this study, we describe a novel and rapid method for the construction of a full-length infectious clone (pPPV). The constructed clone contained an engineered EcoRv site that served as a genetic marker and was shown to be infectious when transfected into a monolayer of PK-15 cells. The rescued virus (rPPV) of the infectious clone was found to be indistinguishable from wild-type virus BQ in terms of its biological properties. The generation of this PPV infectious clone provides a potentially powerful tool with which to elucidate the molecular pathogenesis of PPV.
KeywordsPorcine parvovirus Infectious clone Genetic marker Rescued virus Biological properties
This work was supported by the Agricultural Science and Technology Innovation Program (ASTIP-IAS15), National Natural Science Foundation of China (NO. 31172349 & NO. 31172341) and the National Key Research and Development Program of China (No. 2016YFD0501003 & 2017YFD0502300).
Compliance with ethical standards
Conflicts of interest
All authors declare they have no conflict of interest.
This article does not contain any studies with human participants and the animals studies was approved by the Science Research Department (in charge of animal welfare issue) of the Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (IAS-CAAS) (Beijing, China).
The author unanimously agreed to submit the manuscript to Archives of Virology.
- 1.Mayr A, Mahnel H (1964) Cultivation of hog cholera virus in pig kidney cultures with cytopathogenic effect. Zentralbl Bakteriol Orig 195:157–166Google Scholar
- 2.Cartwright SF, Huck RA (1967) Viruses isolated in association with herd infertility, abortions and stillbirths in pigs. Vet Rec 81:196–197Google Scholar
- 8.Truyen U, Streck AF (2012) Porcine parvovirus. In: Zimmerman J, Karriker L, Ramirez A, Schwartz K, Stevenson G (eds) Diseases of swine 10. Wiley, Oxfort, pp 447–455Google Scholar
- 12.Wang X, Ponnazhagan S, Srivastava A (1996) Rescue and replication of adeno-associated virus type 2 as well as vector DNA sequences from recombinant plasmids containing deletions in the viral inverted terminal repeats: selective encapsidation of viral genomes in progeny virions. J Virol 70:1668–1677Google Scholar
- 21.Berns KI (1990) Parvoviridae and their replication. In: Fields BN, Knipe DM (eds) Virology, 2nd edn. Raven Press Ltd, New York, pp 1743–1760Google Scholar