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Identification and complete genome characterization of human enterovirus 117 from a child with pneumonia in China

  • Juan Du
  • Teng Zhu
  • Lu Zhuang
  • Pan-He Zhang
  • Xiao-Ai Zhang
  • Qing-Bin LuEmail author
  • Wei LiuEmail author
Annotated Sequence Record

Abstract

In this study, human enterovirus C117 (EV-C117) was detected in a 3-month-old boy diagnosed with pneumonia in China. A phylogenetic analysis showed that this strain was genetically closer to the Lithuanian strain than to the USA strain.

Notes

Funding

This study was supported by China Mega-Project for Infectious Diseases Grant (2017ZX10103004, 2018ZX10713002 and 2018ZX10101003), the Natural Science Foundation of China (81703274), and Peking University Medicine Seed Fund for Interdisciplinary Research (BMU2018MX009).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study protocol was reviewed and approved by the Ethics Review Committee of Children’s Hospital of Chongqing Medical University.

Informed consent

We obtained informed consent from the child’s parents.

Supplementary material

705_2019_4196_MOESM1_ESM.tif (1.3 mb)
Supplemental Figure 1 Phylogenetic trees constructed based on 5’UTR, VP4, VP2, VP3, 2A, 2B, 2C, 3A, 3B, 3C, 3D and 3’UTR sequences by the maximum-likelihood method in MEGA 7.0. The isolate from the present study is indicated by a black filled circle. (TIFF 1350 kb)

References

  1. 1.
    Brown B, Oberste MS, Maher K, Pallansch MA (2003) Complete genomic sequencing shows that polioviruses and members of human enterovirus species C are closely related in the noncapsid coding region. J Virol 77:8973–8984CrossRefPubMedCentralGoogle Scholar
  2. 2.
    Daleno C, Piralla A, Scala A, Baldanti F, Usonis V, Principi N, Esposito S (2012) Complete genome sequence of a novel human enterovirus C (HEV-C117) identified in a child with community-acquired pneumonia. J Virol 86:10888–10889CrossRefPubMedCentralGoogle Scholar
  3. 3.
    Daleno C, Piralla A, Usonis V, Scala A, Ivaskevicius R, Baldanti F, Principi N, Esposito S (2012) Novel human enterovirus C infection in child with community acquired pneumonia. Emerg Infect Dis 18:1913–1915CrossRefPubMedCentralGoogle Scholar
  4. 4.
    Gunson RN, Collins TC, Carman WF (2005) Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactions. J Clin Virol 33:341–344CrossRefGoogle Scholar
  5. 5.
    Piralla A, Fiorina L, Daleno C, Esposito S, Baldanti F (2013) Complete genome characterization of enterovirus 104 circulating in Northern Italy shows recombinant origin of the P3 region. Infect Genet Evol 20:111–117CrossRefGoogle Scholar
  6. 6.
    Tiveljung-Lindell A, Rotzen-Ostlund M, Gupta S, Ullstrand R, Grillner L, Zweygberg-Wirgart B, Allander T (2009) Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses. J Med Virol 81:167–175CrossRefGoogle Scholar
  7. 7.
    Van Leer-Buter CC, Poelman R, Borger R, Niesters HG (2016) Newly identified enterovirus c genotypes, identified in the Netherlands through routine sequencing of all enteroviruses detected in clinical materials from 2008 to 2015. J Clin Microbiol 54:2306–2314CrossRefPubMedCentralGoogle Scholar
  8. 8.
    Xiang Z, Tsatsral S, Liu C, Li L, Ren L, Xiao Y, Xie Z, Zhou H, Vernet G, Nymadawa P, Shen K, Wang J (2014) Respiratory infection with enterovirus genotype C117, China and Mongolia. Emerg Infect Dis 20:1075–1077CrossRefPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Laboratorial Science and Technology, School of Public HealthPeking UniversityBeijingPeople’s Republic of China
  2. 2.Vaccine Research Center, School of Public HealthPeking UniversityBeijingPeople’s Republic of China
  3. 3.The Second Affiliated HospitalZhejiang University School of MedicineHangzhouPeople’s Republic of China
  4. 4.The 7th Medical Center of PLA General HospitalBeijingPeople’s Republic of China
  5. 5.State Key Laboratory of Pathogen and BiosecurityBeijing Institute of Microbiology and EpidemiologyBeijingPeople’s Republic of China
  6. 6.Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food SafetyBeijingPeople’s Republic of China

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