Novel bird’s-foot trefoil RNA viruses provide insights into a clade of legume-associated enamoviruses and rhabdoviruses
- 105 Downloads
Here, we report the identification and characterization of two novel viruses associated with bird’s-foot trefoil. Virus sequences related to those of enamoviruses (ssRNA (+); Luteoviridae; Enamovirus) and nucleorhabdoviruses (ssRNA (-); Rhabdoviridae; Nucleorhabdovirus) were detected in Lotus corniculatus transcriptome data. The genome of the tentatively named “bird’s-foot trefoil-associated virus 1” (BFTV-1) is a 13,626-nt-long negative-sense ssRNA. BFTV-1 encodes six predicted gene products in the antigenome orientation in the canonical order 3′-N-P-P3-M-G-L-5′. The genome of the proposed “bird’s-foot trefoil-associated virus 2” (BFTV-2) is 5,736 nt long with a typical 5΄-PO-P1-2-IGS-P3-P5-3′ enamovirus genome structure. Phylogenetic analysis indicated that BFTV-1 is closely related to datura yellow vein nucleorhabdovirus and that BFTV-2 clusters into a monophyletic lineage of legume-associated enamoviruses. This subclade of highly related and co-divergent legume-associated viruses provides insights into the evolutionary history of the enamoviruses.
We would like to express sincere gratitude to the generators of the underlying data used for this work: Dr. Ying Wang and Dr. Zhezhi Wang. By following open access practices and making accessible raw sequence data in public repositories available to the research community, they have promoted the generation of new knowledge and ideas.
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sector.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest
This article does not contain any studies with human participants or animals performed by any of the authors.
- 3.Brown CM, Dinesh-Kumar SP, Miller WA (1996) Local and distant sequences are required for efficient readthrough of the barley yellow dwarf virus PAV coat protein gene stop codon. J Virol 70:5884–5892Google Scholar
- 8.Domier LL (2012) Family Luteoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, ninth report of the international committee on taxonomy of viruses. Elsevier, Oxford, pp 1045–1053Google Scholar
- 9.Escaray FJ, Menendez AB, Garriz A, Pieckenstain FL, Estrella MJ, Castagno LN, Carrasco P, Sanjuan J, Ruiz OA (2012) Ecological and agronomic importance of the plant genus Lotus. Its application in grassland sustainability and the amelioration of constrained and contaminated soils. Plant Science 182:121e133Google Scholar
- 13.Hannaway DB, Myers D (2004) Birdsfoot trefoil (Lotus corniculatus L.). Species selection information system. Oregon State University, CorvallisGoogle Scholar
- 16.McDonald JG, Susuki M (1983) Occurrence of alfalfa mosaic virus in Prince Edward Island. Canadian Plant Disease Survey 63:47–50Google Scholar
- 21.Singh KS, Beadle K, Troczka BJ, Field L, Davies E, Williamson M, Bass C (2018) Extension of partial gene transcripts by iterative mapping of RNA-Seq raw reads. IEEE/ACM Trans Comput Biol Bioinform. https://doi.org/10.1109/tcbb.2018.2865309
- 24.Wu LP, Yang T, Liu HW, Postman J, Li R (2018) Molecular characterization of a novel rhabdovirus infecting blackcurrant identified by high-throughput sequencing. Arch Virol 162:2493–2494Google Scholar