Advertisement

Archives of Virology

, Volume 164, Issue 5, pp 1411–1417 | Cite as

Molecular characterization of bovine noroviruses and neboviruses in Turkey: detection of recombinant strains

  • Ilke Karayel-HaciogluEmail author
  • Feray Alkan
Brief Report
  • 43 Downloads

Abstract

To investigate the molecular epidemiology and genetic diversity of bovine enteric caliciviruses, a total of 167 fecal samples from diarrheic calves were screened. Bovine noroviruses (BoNoVs) and neboviruses were detected in 56 (33.5%) and 37 (22.1%) fecal samples, respectively. Sequences of the RdRp and capsid gene of selected BoNoVs showed that the GIII.1 and GIII.2 genotypes were in circulation in Turkey. Two of the BoNoV strains were identified as recombinant strains (GIII.P1/GIII.2). All examined neboviruses possessed a Nebraska-like RdRp gene. The two nebovirus strains were classified into lineage 4 based on phylogenetic analysis of VP1 amino acid sequences. One of them showed evidence of a recombination event within the S domain. This study is thus the first to reveal the presence of the BoNoV GIII.1 genotype and recombinant strains of BoNoV and neboviruses in Turkey.

Notes

Acknowledgements

This manuscript represents a portion of a thesis submitted by IKH to the Department of Virology, Faculty of Veterinary Medicine, Ankara University, in fulfillment of the requirements for a PhD degree. We thank Dr. Krisztian Banyai for his support and advice as a supervisor during the Fellowship Program (2214A) at the Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary.

Funding

This project was funded by Ankara University Scientific Research Projects Coordination Unit (project no. 13L3338011). IKH was supported by the General National PhD Scholarship Program (2211A) and the International Doctoral Research Fellowship Program (2214A) of the Scientific and Technological Research Council of Turkey (TUBITAK).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.

Supplementary material

705_2019_4186_MOESM1_ESM.jpg (2 mb)
Phylogenetic tree based on the deduced 137-aa sequences of the partial capsid gene sequence of BoNoVs. The tree was constructed by the maximum-likelihood method with bootstrap values calculated for 1000 replicates. The scale bar indicates amino acid substitutions per site. The strains investigated in this study are indicated by black dots. (JPEG 2004 kb)
705_2019_4186_MOESM2_ESM.docx (30 kb)
Supplementary material 2 (DOCX 30 kb)

References

  1. 1.
    Smiley JR, Chang KO, Hayes J et al (2002) Characterization of an enteropathogenic bovine calicivirus representing a potentially new calicivirus genus. J Virol 76:10089–10098CrossRefGoogle Scholar
  2. 2.
    Otto PH, Clarke IN, Lambden PR et al (2011) Infection of calves with bovine norovirus GIII.1 strain jena virus: an experimental model to study the pathogenesis of norovirus ınfection. J Virol 85:12013–12021CrossRefGoogle Scholar
  3. 3.
    Vinjé J (2015) Advances in laboratory methods for detection and typing of norovirus. J Clin Microbiol 53:373–381CrossRefGoogle Scholar
  4. 4.
    Oliver SL, Batten CA, Deng Y et al (2006) Genotype 1 and genotype 2 bovine noroviruses are antigenically distinct but share a cross-reactive epitope with human noroviruses. J Clin Microbiol 44:992–998CrossRefGoogle Scholar
  5. 5.
    Wolf S, Williamson W, Hewitt J et al (2009) Molecular detection of norovirus in sheep and pigs in New Zealand farms. Vet Microbiol 133:184–189CrossRefGoogle Scholar
  6. 6.
    Woode GN, Bridger JC (1978) Isolation of small viruses resembling astroviruses and caliciviruses from acute enteritis of calves. J Med Microbiol 11:441–452CrossRefGoogle Scholar
  7. 7.
    Günther H, Otto P (1987) Diarrhea in young calves. 7. “Zackenvirus”(Jena agent 117/80)—a new diarrhea pathogen in calves. Arch Exp Veterinarmed 41:934Google Scholar
  8. 8.
    Carstens EB (2010) Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2009). Arch Virol 155:133–146CrossRefGoogle Scholar
  9. 9.
    Kaplon J, Guenau E, Asdrubal P et al (2011) Possible novel nebovirus genotype in cattle, France. Emerg Infect Dis 17:1120–1123CrossRefGoogle Scholar
  10. 10.
    D’Mello F, Jervis SM, Edwards PM et al (2009) Heterogeneity in the capsid protein of bovine enteric caliciviruses belonging to a new genus. Virology 387:109–116CrossRefGoogle Scholar
  11. 11.
    Guo Z, He Q, Yue H et al (2018) Genomic characterization of a RdRp-recombinat nebovirus strain with a novel VP1 genotype. Virus Res 251:6–13CrossRefGoogle Scholar
  12. 12.
    Han MG, Smiley JR, Thomas C, Saif LJ (2004) Genetic recombination between two genotypes of genogroup III bovine noroviruses (BoNVs) and capsid sequence diversity among BoNVs and Nebraska-like bovine enteric caliciviruses. J Clin Microbiol 42:5214–5224CrossRefGoogle Scholar
  13. 13.
    Bull RA, Tanaka MM, White PA (2007) Norovirus recombination. J Gen Virol 88:3347–3359CrossRefGoogle Scholar
  14. 14.
    Oliver SL, Brown DW, Green J, Bridger J (2004) A chimeric bovine enteric calicivirus: evidence for genomic recombination in genogroup III of the Norovirus genus of the Caliciviridae. Virology 326:231–239CrossRefGoogle Scholar
  15. 15.
    Di Martino B, Di Profio F, Di Felice E et al (2014) Genetic heterogeneity of bovine noroviruses in Italy. Arch Virol 159:2717–2722CrossRefGoogle Scholar
  16. 16.
    Di Martino B, Di Profio F, Martella V et al (2011) Evidence for recombination in neboviruses. Vet Microbiol 153:367–372CrossRefGoogle Scholar
  17. 17.
    Alkan F, Karayel İ, Catella C et al (2015) Identification of a bovine enteric calicivirus, Kırklareli virus, distantly related to neboviruses, in calves with enteritis in Turkey. J Clin Microbiol 53:3614–3617CrossRefGoogle Scholar
  18. 18.
    Gulacti I, Sozdutmaz I, Isıdan H (2016) Molecular characterization of the bovine noroviruses from diarrhoeic calves in Turkey 2. Turk J Vet Anim Sci 40:1–6CrossRefGoogle Scholar
  19. 19.
    Yilmaz H, Turan N, Altan E et al (2011) First report on the phylogeny of bovine norovirus in Turkey. Arch Virol 156:143–147CrossRefGoogle Scholar
  20. 20.
    Turan T, Işıdan H, Atasoy MO, Irehan B (2018) Detection and molecular analysis of bovine enteric norovirus and nebovirus in Turkey. J Vet Res 62:129–135CrossRefGoogle Scholar
  21. 21.
    Smiley JR, Hoet AE, Traven M et al (2003) Reverse transcription-PCR assays for detection of bovine enteric caliciviruses (BEC) and analysis of the genetic relationships among BEC and human caliciviruses. J Clin Microbiol 41:3089–3099CrossRefGoogle Scholar
  22. 22.
    Park SJ, Jeong C, Yoon SS et al (2006) Detection and characterization of bovine coronaviruses in fecal specimens of adult cattle with diarrhea during the warmer seasons. J Clin Microbiol 44:3178–3188CrossRefGoogle Scholar
  23. 23.
    Larsson A (2014) AliView: a fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30:3276–3278CrossRefGoogle Scholar
  24. 24.
    Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797CrossRefGoogle Scholar
  25. 25.
    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  26. 26.
    Cho YI, Han JI, Wang C et al (2013) Case–control study of microbiological etiology associated with calf diarrhea. Vet Microbiol 166:375–385CrossRefGoogle Scholar
  27. 27.
    Hassine-Zaafrane M, Kaplon J, Sdiri-Loulizi K et al (2012) Molecular prevalence of bovine noroviruses and neboviruses detected in central-eastern Tunisia. Arch Virol 157:1599–1604CrossRefGoogle Scholar
  28. 28.
    Candido M, Alencar ALF, Almeida-Queiroz SR et al (2016) First detection and molecular characterization of Nebovirus in Brazil. Epidemiol Infect 144:1876–1878CrossRefGoogle Scholar
  29. 29.
    Park SI, Jeong C, Park SJ et al (2008) Molecular detection and characterization of unclassified bovine enteric caliciviruses in South Korea. Vet Microbiol 130:371–379CrossRefGoogle Scholar
  30. 30.
    Oliver SL, Asobayire E, Dastjerdi AM, Bridger JC (2006) Genomic characterization of the unclassified bovine enteric virus Newbury agent-1 (Newbury1) endorses a new genus in the family Caliciviridae. Virology 350:240–250CrossRefGoogle Scholar
  31. 31.
    Pourasgari F, Kaplon J, Sanchooli A et al (2018) Molecular prevalence of bovine noroviruses and neboviruses in newborn calves in Iran. Arch Virol 163:1271–1277CrossRefGoogle Scholar
  32. 32.
    Ferragut F, Vega CG, Mauroy A et al (2016) Molecular detection of bovine Noroviruses in Argentinean dairy calves: circulation of a tentative new genotype. Infect Genet Evol 40:144–150CrossRefGoogle Scholar
  33. 33.
    Park SI, Jeong C, Kim HH et al (2007) Molecular epidemiology of bovine noroviruses in South Korea. Vet Microbiol 124:125–133CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of VirologyAnkara University, Faculty of Veterinary MedicineAnkaraTurkey

Personalised recommendations