Biological characterization and pathogenicity of a newly isolated Chinese highly virulent genotype GIIa porcine epidemic diarrhea virus strain
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Since 2010, continual outbreaks of highly virulent variants of porcine epidemic diarrhea virus (PEDV) belonging to genotype GII have led to serious economic losses for the Chinese swine industry. To better understand the biological characteristics and pathogenicity of the current prevalent Chinese PEDV field strains, in this study, a highly virulent Chinese genotype GIIa PEDV strain, CH/HBXT/2018, was isolated and serially propagated using Vero cells. Sequencing and phylogenetic analysis showed that strain CH/HBXT/2018 contained novel insertion and deletion mutations in the S gene region relative to the classical strain and belonged to the genotype GIIa, similar to other recently isolated PEDV strains from China and the United States. Pig infection studies indicated that the CH/HBXT/2018 strain was highly virulent in suckling piglets, and the median pig diarrhea dose (PDD50) was 8.63 log10PDD50/3 mL at 7 days postinfection (DPI). The results of the present study are important for future PEDV challenge studies and the development of new PEDV vaccines based on prevalent field strains for the prevention and control of PED in China.
This study was supported by the National Natural Science Foundation of China (Grant no. 31602095), the National Key Research and Development Program (2016YFD0501505), the China Agriculture Research System (CARS-35) and the Central Public Interest Scientific Institution Basal Research Fund (Y2016CG23).
XL, YW and YZ conceived and designed the experiments. XL wrote the manuscript and analyzed the data. XL performed the sample collection. XL, LZ, QZ, PZ, YF, ZD, DZ, WL, and JF performed the experiments. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
All piglets used in the present study were humanely bred during the experiment and euthanized at the end of the experiment. Animal care and use protocols were reviewed and approved by the Institutional Animal Use and Care Committee of Lanzhou Veterinary Research Institute.
- 11.Puranaveja S, Poolperm P, Lertwatcharasarakul P, Kesdaengsakonwut S, Boonsoongnern A, Urairong K, Kitikoon P, Choojai P, Kedkovid R, Teankum K, Thanawongnuwech R (2009) Chinese-like strain of porcine epidemic diarrhea virus, Thailand. Emerg Infect Dis 15(7):1112–1115. https://doi.org/10.3201/eid1507.081256 CrossRefGoogle Scholar
- 13.Lara-Romero R, Gomez-Nunez L, Cerriteno-Sanchez JL, Marquez-Valdelamar L, Mendoza-Elvira S, Ramirez-Mendoza H, Rivera-Benitez JF (2018) Molecular characterization of the spike gene of the porcine epidemic diarrhea virus in Mexico, 2013–2016. Virus Genes 54(2):215–224. https://doi.org/10.1007/s11262-017-1528-x CrossRefGoogle Scholar
- 14.Jarvis MC, Lam HC, Zhang Y, Wang L, Hesse RA, Hause BM, Vlasova A, Wang Q, Zhang J, Nelson MI, Murtaugh MP, Marthaler D (2016) Genomic and evolutionary inferences between American and global strains of porcine epidemic diarrhea virus. Prev Vet Med 123:175–184. https://doi.org/10.1016/j.prevetmed.2015.10.020 CrossRefGoogle Scholar
- 16.Zheng FM, Huo JY, Zhao J, Chang HT, Wang XM, Chen L, Wang CQ (2013) Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus field strains in central China during 2010–2012 outbreaks. Bing du xue bao Chin J Virol 29(2):197–205Google Scholar
- 24.Oka T, Saif LJ, Marthaler D, Esseili MA, Meulia T, Lin CM, Vlasova AN, Jung K, Zhang Y, Wang Q (2014) Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene. Vet Microbiol 173(3–4):258–269. https://doi.org/10.1016/j.vetmic.2014.08.012 CrossRefGoogle Scholar