Genomic characterization of a novel recombinant porcine astrovirus isolated in northeastern China
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Porcine astroviruses (PAstVs), are widely distributed viruses that are highly prevalent in swine herds. In this study, a novel type 4 porcine astrovirus strain (designated as PAstV4/Tianjin/2018) was identified in a fecal sample from a diarrheal piglet in Tianjin, China and its complete genomic sequence was determined by RT-PCR. Sequence analysis showed that this strain had a capsid protein with a highly variable C-terminal domain, a typical ribosomal frameshifting signal, and a conserved subgenomic promoter sequence. Recombination analysis indicated that PAstV4/Tianjin/2018 was a novel recombinant strain, and a recombination breakpoint was identified at nt position 4220 of the genome. The novel recombinant porcine astrovirus identified in China will be useful for understanding the origin, genetic diversity, and evolution of enteric viruses.
This work was supported by the National Key Research Project of China (2016YFD050030), the underprop project of Tianjin Science and Technology Committee in China (16YFZCNC00640 and 17JCZDJC33900), and College Students’ Innovation and Entrepreneurship Training Project (201810056103).
Compliance with ethical standards
Animal and human rights statement
Animal experiments were performed in compliance with the regulations set by Tianjin University Institutional Animal Care and Use Committee and were approved by the veterinarian authorities of Tianjin Animal Inspection Institute. This study was carried out in strict accordance with the guidelines in the Guide for the Care and Use of Laboratory Animals in China.
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