Viral metagenomics reveals significant viruses in the genital tract of apparently healthy dairy cows
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The virome in genital tract secretion samples collected from 80 dairy cattle in Shanghai, China, was characterized. Viruses detected included members of the families Papillomaviridae, Polyomaviridae, Hepeviridae, Parvoviridae, Astroviridae, Picornaviridae, and Picobirnaviridae. A member of a new species within the genus Dyoxipapillomavirus and six circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) viral genomes were fully sequenced and phylogenetically analyzed. The prevalence of bovine polyomaviruses 1 and 2 was measured by PCR to be 10% (8/80) and 6.25% (5/80), respectively. PCR screening also indicated that the novel papillomavirus ujs-21015 and bovine herpesvirus 6 were present in three and two out of the 80 samples, respectively.
This work was partly supported by National Key Research and Development Programs of China no. 2017YFC1200201, Jiangsu Provincial Key Research and Development Projects no. BE2017693, National Natural Science Foundation of China nos. 81741062 and 31572525, Taizhou Science and Technology Support Project no. TS201623, Key Research and Development Plan of Zhenjiang no. SH2016060, and Blood Systems Research Institute.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This study did not include experiments with human participants or animals performed by any of the authors.
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