Archives of Virology

, Volume 164, Issue 4, pp 1059–1067 | Cite as

Viral metagenomics reveals significant viruses in the genital tract of apparently healthy dairy cows

  • Yu Ling
  • Xiaodan Zhang
  • Guilan Qi
  • Shixing Yang
  • Li Jingjiao
  • Quan Shen
  • Xiaochun Wang
  • Li Cui
  • Xiuguo HuaEmail author
  • Xutao Deng
  • Eric Delwart
  • Wen ZhangEmail author
Original Article


The virome in genital tract secretion samples collected from 80 dairy cattle in Shanghai, China, was characterized. Viruses detected included members of the families Papillomaviridae, Polyomaviridae, Hepeviridae, Parvoviridae, Astroviridae, Picornaviridae, and Picobirnaviridae. A member of a new species within the genus Dyoxipapillomavirus and six circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) viral genomes were fully sequenced and phylogenetically analyzed. The prevalence of bovine polyomaviruses 1 and 2 was measured by PCR to be 10% (8/80) and 6.25% (5/80), respectively. PCR screening also indicated that the novel papillomavirus ujs-21015 and bovine herpesvirus 6 were present in three and two out of the 80 samples, respectively.



This work was partly supported by National Key Research and Development Programs of China no. 2017YFC1200201, Jiangsu Provincial Key Research and Development Projects no. BE2017693, National Natural Science Foundation of China nos. 81741062 and 31572525, Taizhou Science and Technology Support Project no. TS201623, Key Research and Development Plan of Zhenjiang no. SH2016060, and Blood Systems Research Institute.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study did not include experiments with human participants or animals performed by any of the authors.

Supplementary material

705_2019_4158_MOESM1_ESM.doc (34 kb)
Supplementary material 1 (DOC 33 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Yu Ling
    • 1
  • Xiaodan Zhang
    • 2
  • Guilan Qi
    • 3
  • Shixing Yang
    • 1
  • Li Jingjiao
    • 4
  • Quan Shen
    • 1
  • Xiaochun Wang
    • 1
  • Li Cui
    • 4
  • Xiuguo Hua
    • 4
    Email author
  • Xutao Deng
    • 5
  • Eric Delwart
    • 5
    • 6
  • Wen Zhang
    • 1
    Email author
  1. 1.Department of Microbiology, School of MedicineJiangsu UniversityZhenjiangChina
  2. 2.Zhenjiang Center for Disease Prevention and ControlZhenjiangChina
  3. 3.Institute of Animal Husbandry, Chengdu Academy of Agriculture and Forestry SciencesChengduChina
  4. 4.School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghaiChina
  5. 5.Blood Systems Research InstituteSan FranciscoUSA
  6. 6.Department of Laboratory MedicineUniversity of California, San FranciscoSan FranciscoUSA

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