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Escherichia coli bacteriophage Gostya9, representing a new species within the genus T5virus

  • Alla K. Golomidova
  • Eugene E. Kulikov
  • Vladislav V. Babenko
  • Pavel A. Ivanov
  • Nikolai S. Prokhorov
  • Andrey V. Letarov
Annotated Sequence Record
  • 9 Downloads

Abstract

Escherichia coli bacteriophage Gostya9 (genus T5virus) was isolated from horse feces collected in Moscow, Russia, in 2013. This phage was associated in a single plaque with the previously reported phage 9g and was subsequently purified. Analysis of the complete genomic sequence of Gostya9 revealed that it is closely related to the T5-like bacteriophage DT57C, which had been isolated at the same location in 2007. These two viruses share 79.5% nucleotide sequence identity, which is below the 95% threshold applied currently to demarcate bacteriophage species. The most significant features distinguishing Gostya9 from DT57C include 1) the presence of one long tail fiber protein gene, 122c (ltf), instead of the two genes, ltfA and ltfB, that are present in DT57C; 2) the absence of the gene for the receptor-blocking lytic conversion lipoprotein precursor llp; and 3) the divergence of the receptor-recognition protein, pb5, which is only distantly related at the amino acid sequence level. The observed features of the Gostya9 adsorption apparatus are suggestive of a possible novel specificity for the final receptor and make this phage interesting for possible direct application in phage therapy of E. coli infections or as a source of receptor-recognition protein for engineering new phage specificities.

Notes

Acknowledgements

We are grateful to Prof. Elizabeth M. Kutter from Evergreen State College, Olympia, USA, for critical reading and linguistic corrections of the manuscript. This work was supported by RSF grant no. 15-15-00134P.

Compliance with ethical standards

All research was conducted in compliance with the national ethical standard code.

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Federal State Institution «Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences», Winogradsky Institute of MicrobiologyMoscowRussian Federation
  2. 2.Moscow Institute of Physics and TechnologyMoscowRussian Federation
  3. 3.Federal Medical Biological Agency, Federal Research and Clinical Center of Physical-Chemical MedicineMoscowRussian Federation
  4. 4.The Mental Health Research CenterMoscowRussia
  5. 5.Department of Biochemistry and Molecular BiologySealy Center for Structural Biology and Molecular Biophysics, University of Texas, Medical BranchGalvestonUSA
  6. 6.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia

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