Complete genome sequence analysis of canine bocavirus 1 identified for the first time in domestic cats
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In this study, we investigated the presence of canine bocaviruses (CBoVs) in fecal samples from 105 cats with diarrhea and 92 asymptomatic cats in northeast China. One fecal sample, 17CC0312, collected from an asymptomatic cat, was found to be positive for canine bocavirus 1 (CBoV1). The nearly complete genome of this virus was cloned and sequenced. The viral genome was 5,069 nucleotides (nt) in length and combined four open reading frames (ORFs) in the order 5′-NS1-ORF4-NP1-VP1/VP2-3′. The 17CC0312 virus shared more than 90.3% nucleotide sequence identity with CBoV1 reference sequences and was placed within the CBoV1 group in a phylogenetic tree based on complete genome sequences. Further phylogenetic analysis based on the deduced amino acid sequence of the VP2 gene showed that this feline CBoV1 strain belongs to CBoV1 lineage 3. These data provide the first molecular evidence of the presence of CBoV1 in a domestic cat and suggest that cats might be carriers of CBoV1.
This study was supported by the Research Project of the National Key Research and Development Plan of China (Grant no. 2016YFD0501002). This study was also funded by the Special Public Welfare Industry Research of the Ministry of Agriculture of China (no. 201303042) and the National Key R&D Program of China (no. 2017YFD0501703).
Compliance with ethical standards
Conflict of interest
All authors declare no conflict of interest.
All animal experiments conformed to the guidelines and regulatory requirements established by the Animal Care and Use Committee of Jilin Agricultural University, Jilin Province, China. The owner’s consent was obtained to collect samples from healthy and diarrheal cats.
- 1.Binn LN, Lazar EC, Eddy GA, Kajima M (1970) Recovery and characterization of a minute virus of canines. Infect Immun 1:503–508Google Scholar
- 6.Guo D, Wang Z, Yao S, Li C, Geng Y, Wang E, Zhao X, Su M, Wei S, Wang X, Feng L, Chang YF, Sun D (2016) Epidemiological investigation reveals genetic diversity and high co-infection rate of canine bocavirus strains circulating in Heilongjiang province, Northeast China. Res Vet Sci 106:7–13CrossRefGoogle Scholar
- 20.Storz J, Leary JJ, Carlson JH, Bates RC (1978) Parvoviruses associated with diarrhea in calves. J Am Vet Med Assoc 173:624–627Google Scholar