Complete genome sequence of a putative novel potyvirus isolated from Platycodon grandiflorum
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The complete 9,556-nt genomic sequence of a putative new potyvirus, tentatively named “platycodon mild mottle virus” (PlaMMV), infecting Platycodon grandiflorum was determined (GenBank accession no. MH779625). A single large open reading frame (ORF; nt 121-9360, 3079 aa) encoding a polyprotein that was predicted to be cleaved into 10 mature proteins was detected. By comparison with other potyviruses, nine cleavage sites and conserved domains/motifs were identified. PlaMMV also has a highly conserved GA6 motif (nt 2857-2863) that could enable a small overlapping ORF (PIPO) embedded within the P3 cistron to be translated by a viral polymerase slippage mechanism. A BLAST analysis showed that the complete PlaMMV nucleotide sequence shared 70% identity (40% query coverage) with carrot thin leaf virus (JX156434) and that the complete amino acid sequence of the PlaMMV polyprotein shared 54% identity (98% query coverage) with Thunberg fritillary mosaic virus (CAI59123). These results suggest that PlaMMV is a new member of the genus Potyvirus in the family Potyviridae.
This work was supported by the KRIBB Research Initiative Program, funded by the Ministry of Science and ICT, Republic of Korea.
Compliance with ethical standards
This study was funded by KRIBB (Ministry of Science and ICT), Republic of Korea.
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
The dataset generated during the current study is available in the GenBank database (accession no. MH779625).