Characterization of the L genome segment of an orthohantavirus isolated from Niviventer confucianus
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We sequenced and analyzed the L segment of the RNA genome of Hantaan virus (HTNV) strain NC167. This segment is 6,533 nucleotides in length and contains a single open reading frame (ORF) of 6,456 nucleotides in the antigenome sense that encodes the viral RNA-dependent RNA polymerase, which is 2,153 amino acids long with a predicted molecular mass of 246 kDa. The 5′ terminus of the viral RNA was found to contain the expected sequences that are conserved in orthohantaviruses. According to the phylogenetics and levels of sequence similarity, the L segment of HTNV NC167 is similar to but clearly distinct from the L segments of other orthohantaviruses.
This study was supported by the Natural Science Foundation of Shaanxi Province, China (Grant no. 2007K11-01).
Compliance with ethical standards
Conflict of interest
We declare that we have no competing interests that might have influenced the performance or presentation of the work described in this report.
This study was performed in accordance with the Declaration of Helsinki and was approved by the Research Ethics Committee of Xi’an Jiaotong University, China.