Thailand orthohantavirus infection in patients with chronic kidney disease of unknown aetiology in Sri Lanka
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Chronic kidney disease of unknown aetiology (CKDu) reported in Sri Lanka and other countries is a mysterious and serious disease. Recently, we reported a high seroprevalence of antibodies to a hantavirus antigen among CKDu patients in Girandurukotte, Badulla district, Sri Lanka. However, the type of hantavirus with which the residents were infected was not determined. In this study, a total of 89 seropositive sera were examined to identify their serotypes using an indirect immunofluorescent antibody assay, a truncated-N-protein-based enzyme-linked immunosorbent assay, and a cross-neutralization test. These results indicated that the residents in this area were frequently infected with Thailand orthohantavirus or an antigenically related virus.
We express sincere thanks to Dr. Akio Koizumi of Kyoto University Graduate School of Medicine for helpful discussions.
Compliance with ethical standards
The study was approved by the Institutional Ethical Review Committee, Faculty of Medicine, University of Peradeniya, Sri Lanka (2016/EC/64), and was also approved by the Ethical Review Committee of the Faculty of Medicine, Hokkaido University, Japan (M17-023). Patient/participant consent was obtained prior to recruitment into the study.
Conflict of interest
We declare that we have no conflict of interest.
- 4.Fulhorst CF, Monroe MC, Salas RA, Duno G, Utrera A, Ksiazek TG, Nichol ST, de Manzione NM, Tovar D, Tesh RB (1997) Isolation, characterization and geographic distribution of Cano Delgadito virus, a newly discovered South American hantavirus (family Bunyaviridae). Virus Res 51:159–171CrossRefGoogle Scholar
- 5.Gamage CD, Yasuda SP, Nishio S, Kularatne SA, Weerakoon K, Rajapakse J, Nwafor-Okoli C, Lee RB, Obayashi Y, Yoshimatsu K, Arikawa J, Tamashiro H (2011) Serological evidence of Thailand virus-related hantavirus infection among suspected leptospirosis patients in Kandy, Sri Lanka. Jpn J Infect Dis 64:72–75PubMedGoogle Scholar
- 7.Jameson LJ, Taori SK, Atkinson B, Levick P, Featherstone CA, van der Burgt G, McCarthy N, Hart J, Osborne JC, Walsh AL, Brooks TJ, Hewson R (2013) Pet rats as a source of hantavirus in England and Wales, 2013. Eurosurveillance 18:e57538Google Scholar
- 10.Koma T, Yoshimatsu K, Pini N, Safronetz D, Taruishi M, Levis S, Endo R, Shimizu K, Yasuda SP, Ebihara H, Feldmann H, Enria D, Arikawa J (2010) Truncated hantavirus nucleocapsid proteins for serotyping Sin Nombre, Andes, and Laguna Negra hantavirus infections in humans and rodents. J Clin Microbiol 48:1635–1642CrossRefGoogle Scholar
- 15.Palihawadana P (2009) Research programme for chronic kidney disease of unknown aetiology in Sri Lanka. WER Sri Lanka 36(49):1–4Google Scholar
- 16.Pattamadilok S, Lee BH, Kumperasart S, Yoshimatsu K, Okumura M, Nakamura I, Araki K, Khoprasert Y, Dangsupa P, Panlar P, Jandrig B, Kruger DH, Klempa B, Jakel T, Schmidt J, Ulrich R, Kariwa H, Arikawa J (2006) Geographical distribution of hantaviruses in Thailand and potential human health significance of Thailand virus. Am J Trop Med Hyg 75:994–1002CrossRefGoogle Scholar
- 26.Yoshimatsu K, Arikawa J, Kariwa H (1993) Application of a recombinant baculovirus expressing hantavirus nucleocapsid protein as a diagnostic antigen in IFA test: cross reactivities among 3 serotypes of hantavirus which causes hemorrhagic fever with renal syndrome (HFRS). J Vet Med Sci 55:1047–1050CrossRefGoogle Scholar