Complete genome sequence of C130_2, a novel myovirus infecting pathogenic Escherichia coli and Shigella strains
The genome sequence of a novel virulent bacteriophage, named “ C130_2”, that is morphologically a member of the family Myoviridae is reported. The 41,775-base-pair double-stranded DNA genome of C130_2 contains 59 ORFs but exhibits overall low sequence similarity to bacteriophage genomes for which sequences are publicly available. Phylogenetic analysis indicated that C130_2 represents a new phage type. C130_2 could be propagated well on enterohemorrhagic Escherichia coli (EHEC) O157:H7 and other pathogenic E. coli strains, as well as on strains of various Shigella species.
This study was supported by the Hungarian National Research, Development and Innovative Office (NKFIH, Grant 124335), 7th EU Framework Programme PROMISE project (Project No. 265877) and by grants to the German Center of Infection Research (DZIF, site Giessen-Marburg-Langen), through the German Federal Ministry of Education and Research, Grant # TI06.001 and 8032808811 to TC. We thank Christina Gerstmann for excellent technical assistance. Domonkos Sváb was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences.
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