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Decrease in the expression level of the gene encoding the putative Bombyx mori bidensovirus receptor during virus infection

  • Katsuhiko Ito
  • Takeshi Fujii
  • Takeshi Yokoyama
  • Keiko Kadono-Okuda
Original Article

Abstract

Bombyx mori bidensovirus (BmBDV) is a pathogen that replicates only in the midgut columnar cells of silkworms, causing fatal disease. Resistance to BmBDV, which does not depend on the viral dose, is determined by a single gene, nsd-2 (resistance gene). Previously, we identified nsd-2 by positional cloning using B. mori genome information and found that this gene encodes a putative amino acid transporter that may function as a receptor for BmBDV. In this study, to understand the relationship between BmBDV and the putative virus receptor, we performed expression analysis of +nsd−2 (allele of nsd-2; susceptibility gene) after virus infection. Quantitative RT-PCR analysis using total RNA isolated from the midgut of an uninfected and a virus-infected silkworm revealed no change in the expression levels of +nsd−2 in the uninfected silkworm, whereas the expression levels of +nsd−2 drastically decreased in the virus-infected silkworm. Moreover, comparison of the expression pattern between the BmBDV-derived transcript and +nsd−2 revealed that the expression level of +nsd−2 decreased with an increase in the virus-derived transcript. In addition, expression analysis of 26 genes encoding other transporters in the midgut demonstrated that the expression levels of three other genes also decreased similarly to the decrease of the expression levels of +nsd−2 after virus infection. Thus, our results suggest that some transporters, including +nsd−2, are affected by BmBDV infection.

Abbreviations

nsd-2

Non-susceptibility to BmDNV-2

nsd-Z

Non-susceptibility to Zhenjiang (China) strain of BmDNV

RT-PCR

Reverse transcription polymerase chain reaction

Notes

Acknowledegments

This work was supported, in part, by JSPS Grant-in-Aid for Young Scientists (B) 15K18807 (to KI).

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interests.

Supplementary material

705_2018_4017_MOESM1_ESM.pdf (1.1 mb)
Supplementary material 1 (PDF 1083 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Science of Biological ProductionTokyo University of Agriculture and TechnologyFuchuJapan
  2. 2.Division of BiotechnologyInstitute of Agrobiological Sciences, National Agriculture and Food Research OrganizationTsukubaJapan

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