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Archives of Virology

, Volume 163, Issue 11, pp 3191–3194 | Cite as

Complete genome sequence of a novel virus, classifiable within the Potyviridae family, which infects passion fruit (Passiflora edulis)

  • Sara Jover-Gil
  • Avital Beeri
  • Patricia Fresnillo
  • Alon Samach
  • Héctor Candela
Annotated Sequence Record

Abstract

We report the complete nucleotide sequence of a new member of the Potyviridae family isolated from passion fruit plants grown in Israel, called Passiflora edulis symptomless virus (PeSV). The PeSV genome is 9,928 nucleotides long and encodes a 3,173 amino acids polyprotein that is predicted to be proteolytically cleaved into 10 mature peptides. Our phylogenetic analysis shows that PeSV represents a new species, and is most closely related to rose yellow mosaic virus (RoYMV). According to currently accepted criteria for genus demarcation, both viruses should be assigned as representative isolates of new species in the recently approved genus, Roymovirus, in the Potyviridae family.

Notes

Funding

This study was partly funded by Grant number 837-0147-14 from the Office of the Chief Scientist, Ministry of Agriculture and Rural Development of Israel, to A. S.

Compliance with ethical standards

Conflict of interest

All authors declare that no conflict of interest exists.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3983_MOESM1_ESM.pdf (95 kb)
Supplemental Figure 1. Matrix of nucleotide sequence identity values for selected members of the Potyviridae family. (PDF 95 kb)

References

  1. 1.
    Adams MJ, Antoniw JF, Beaudoin F (2005) Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol Plant Pathol 6:471–487CrossRefPubMedGoogle Scholar
  2. 2.
    Adams MJ, Antoniw JF, Fauquet CM (2005) Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150:459–479CrossRefPubMedGoogle Scholar
  3. 3.
    Blumenfeld A, Shaya F (2004) Sweet dream. Yevul See 6:40–41Google Scholar
  4. 4.
    Iwai H, Yamashita Y, Nishi N, Nakamura M (2006) The potyvirus associated with the dappled fruit of Passiflora edulis in Kagoshima prefecture, Japan is the third strain of the proposed new species East Asian Passiflora virus (EAPV) phylogenetically distinguished from strains of Passion fruit woodiness virus. Arch Virol 151:811–818CrossRefPubMedGoogle Scholar
  5. 5.
    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Langmead B, Salzberg SL (2012) Fast gapped-read alignment with Bowtie 2. Nat Meth 9:357–359CrossRefGoogle Scholar
  7. 7.
    Mollov D, Lockhart B, Zlesak D (2013) Complete nucleotide sequence of rose yellow mosaic virus, a novel member of the family Potyviridae. Arch Virol 158:1917–1923CrossRefPubMedGoogle Scholar
  8. 8.
    Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New YorkGoogle Scholar
  9. 9.
    Olspert A, Chung BY, Atkins JF, Carr JP, Firth AE (2015) Transcriptional slippage in the positive-sense RNA virus family Potyviridae. EMBO Rep 16:995–1004CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Schulz MH, Zerbino DR, Vingron M, Birney E (2012) Oases: robust de novo RNA-seq assembly across the dynamic range of expression levels. Bioinformatics 28:1086–1092CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Seo JK, Kim MK, Kwak HR, Kim JS, Choi HS (2017) Complete genome sequence of longan witches’ broom-associated virus, a novel member of the family Potyviridae. Arch Virol 162:2885–2889CrossRefPubMedGoogle Scholar
  12. 12.
    Spiegel S, Zeidan M, Sobolev I, Beckelman Y, Holdengreber V, Tam Y, Joseph MB, Lipsker Z, Gera A (2007) The complete nucleotide sequence of Passiflora latent virus and its phylogenetic relationship to other carlaviruses. Arch Virol 152:181–189CrossRefPubMedGoogle Scholar
  13. 13.
    Ward CW (2017) Is it time to retire the genus Rymovirus from the family Potyviridae? Arch Virol 162:2175–2179CrossRefPubMedGoogle Scholar
  14. 14.
    Wylie SJ, Adams A, Chalam C, Kreuze J, López-Moya JJ, Ohshima K, Praveen S, Rabenstein F, Stenger D, Wang A, Serbini FM, ICTV Report Consortium (2017) ICTV virus taxonomy profile: Potyviridae. J Gen Virol 98:352–354CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Instituto de BioingenieríaUniversidad Miguel HernándezElcheSpain
  2. 2.The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and EnvironmentThe Hebrew University of JerusalemRehovotIsrael

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