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Archives of Virology

, Volume 163, Issue 11, pp 3195–3197 | Cite as

Complete genome analysis of bacteriophage AsXd-1, a new member of the genus Hk97virus, family Siphoviridae

  • Zhenlong Yang
  • Shengjian Yuan
  • Ling Chen
  • Quan Liu
  • Haoran Zhang
  • Yingfei Ma
  • Ting Wei
  • Shuqiang Huang
Annotated Sequence Record

Abstract

AsXd-1, a bacteriophage that infects Aeromonas salmonicida, was isolated from the wastewater of a seafood market in Shenzhen, China. The 39,014-bp genome of this phage contains 52 open reading frames (ORFs), 30 of which were found to be homologous to reference sequences that putatively encode functional phage proteins. Nine out of the remaining 22 ORFs with unknown functions were unique to AsXd-1. Gene annotation suggests that AsXd-1 has both lysogenic and lytic life cycles. Furthermore, both phylogenetic analysis based on the large subunit of terminase and genome sequence comparisons show that AsXd-1 is closely related to phages belonging to the genus Hk97virus. We thus propose AsXd-1 as a new member of the genus Hk97virus within the family Siphoviridae.

Notes

Acknowledgements

We are grateful to J. Feng from the Institute of Microbiology of the Chinese Academy of Sciences for providing bacterial strains. This study was funded by the Shenzhen Science and Technology Innovation Committee (JCYJ20170413153329565, JCYJ20160229201759414, JCYJ20150521144320992, JSGG20160229145252927, KQJSCX20160301144623), the Engineering Laboratory for Automated Manufacturing of Therapeutic Synthetic Microbes (Shenzhen Development and Reform Commission No. [2016]1194), the Shenzhen Peacock Team Project (KQTD2015033117210153, KQTD2016112915000294), the National Natural Science Foundation of China (31770111, 31500104, 31570115), the Research Instrument Development Project from Chinese Academy of Sciences (YJKYYQ20170063), and the Natural Science Foundation of Guangdong Province (2015A030313745, 2015A030310123, 2015A030310471).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_3977_MOESM1_ESM.doc (50 kb)
Supplementary material 1 (DOC 50 kb)
705_2018_3977_MOESM2_ESM.pdf (2.8 mb)
Supplementary material 2 (PDF 2895 kb)
705_2018_3977_MOESM3_ESM.xlsx (15 kb)
Supplementary material 3 (XLSX 15 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Zhenlong Yang
    • 1
  • Shengjian Yuan
    • 1
  • Ling Chen
    • 1
  • Quan Liu
    • 1
    • 2
  • Haoran Zhang
    • 1
  • Yingfei Ma
    • 1
  • Ting Wei
    • 1
  • Shuqiang Huang
    • 1
  1. 1.Institute of Synthetic Biology, Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenChina
  2. 2.Department of Food Science and Engineering, College of Chemistry and Chemical EngineeringShenzhen UniversityShenzhenChina

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