Advertisement

Archives of Virology

, Volume 163, Issue 11, pp 3207–3210 | Cite as

Full genome sequence of a polyvalent bacteriophage infecting strains of Shigella, Salmonella, and Escherichia

  • Alaaeldin Mohamed Saad
  • Ahmed Askora
  • Takeru Kawasaki
  • Makoto Fujie
  • Takashi Yamada
Annotated Sequence Record

Abstract

A novel lytic bacteriophage, Escherichia phage EcS1, was isolated from sewage samples collected in Higashi-Hiroshima, Japan. The complete genome sequence of EcS1 was determined using the Illumina Miseq System. The whole genome of EcS1 was found to be 175,437 bp in length with a mean G+C content of 37.8%. A total of 295 genes were identified as structural, functional, and hypothetical genes. BLAST analysis of the EcS1 genomic sequence revealed the highest identity (79%; query cover of 73–74%) to three T4-related phages that infect Serratia sp. ATCC 39006. Host range experiments revealed that EcS1 has lytic effects on three pathogenic strains of Shigella spp. and a pathogenic strain of Salmonella enterica as well as on E. coli strains. However, two strains of Serratia marcescens showed resistance to this phage. Phylogenetic trees for phage tail fiber protein sequences revealed that EcS1 is closely related to Enterobacteriaceae-infecting phages. Thus, EcS1 is a novel phage that infects several pathogenic strains of the family Enterobacteriaceae.

Notes

Acknowledgements

The authors are grateful to T. Shimamoto and A. M. Soliman for host range experiments. This study was supported by the Japan Society for the Promotion of Science, KAKENHI (Grant number 15H04477). We also thank Katie Oakley, Ph.D., from Edanz Group (http://www.edanzediting.com/ac) for editing a draft of this manuscript.

Funding

This study was supported by the Japan Society for the Promotion of Science, KAKENHI (Grant number 15H04477).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals.

Supplementary material

705_2018_3971_MOESM1_ESM.pdf (4.6 mb)
Supplementary material 1 (PDF 4726 kb)

References

  1. 1.
    Starling S (2017) Shigella sonnei has the edge. Nat Rev Microbiol 15:450–451.  https://doi.org/10.1038/nrmicro.2017.74 CrossRefPubMedGoogle Scholar
  2. 2.
    Nikfar R, Shamsizadeh A, Darbor M, Khaghani S, Moghaddam M (2017) A study of prevalence of Shigella species and antimicrobial resistance patterns in paediatric medical center, Ahvaz, Iran. Iran J Microbiol 9(5):277–282PubMedPubMedCentralGoogle Scholar
  3. 3.
    Ansari S, Nepal HP, Gautam R, Shrestha S, Neopane P, Gurung G, Chapagain ML (2015) Community acquired multi-drug resistant clinical isolates of Escherichia coli in a tertiary care center of Nepal. Antimicrob Resist Infect Control 4:15.  https://doi.org/10.1186/s13756-015-0059-2 CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Bao H, Zhang P, Zhang H, Zhou Y, Zhang L, Wang R (2015) Bio-control of Salmonella Enteritidis in foods using bacteriophages. Viruses 7:4836–4853.  https://doi.org/10.3390/v7082847 CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Zhou Y, Liang Y, Lynch KH, Dennis JJ, Wishart DS (2011) PHAST: a fast phage search tool. Nucleic Acids Res 39:W347–W352CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874.  https://doi.org/10.1093/molbev/msw054 CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Miller ES, Kutter E, Mosig G, Arisaka F, Kunisawa T, Ruger W (2003) Bacteriophage T4 genome. Microbiol Mol Biol Rev 67(1):86–156CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Molecular Biotechnology, Graduate School of Advanced Sciences of MatterHiroshima UniversityHigashi-HiroshimaJapan
  2. 2.Department of Zoonoses, Faculty of Veterinary MedicineZagazig UniversityZagazigEgypt
  3. 3.Department of Microbiology, Faculty of ScienceZagazig UniversityZagazigEgypt
  4. 4.Hiroshima Study CenterThe Open University of JapanHiroshimaJapan

Personalised recommendations