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Archives of Virology

, Volume 163, Issue 11, pp 3119–3124 | Cite as

Cholesterol is important for the entry process of porcine deltacoronavirus

  • Ji Hyun Jeon
  • Changhee Lee
Brief Report

Abstract

The present study was conducted to examine whether cellular and/or viral cholesterol levels play a role in porcine deltacoronavirus (PDCoV) replication. Our results showed that depletion of cholesterol from cells or virions by treating them with methyl-β-cyclodextrin (MβCD) diminished PDCoV infection in a dose-dependent manner. The addition of exogenous cholesterol to MβCD-treated cells or virions moderately restored PDCoV infectivity. Furthermore, the pharmacological sequestration of cellular or viral cholesterol efficiently blocked both virus attachment and internalization. Taken together, the current data indicate that the cholesterol present in the cell membrane and viral envelope contributes to PDCoV replication by acting as a key component in viral entry.

Notes

Funding

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015R1D1A1A09057406).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.

Supplementary material

705_2018_3967_MOESM1_ESM.pdf (15.7 mb)
Supplementary material 1 (PDF 16045 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Animal Virology Laboratory, BK21 Plus KNU Creative BioResearch Group, School of Life Sciences, College of Natural SciencesKyungpook National UniversityDaeguRepublic of Korea

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