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Archives of Virology

, Volume 163, Issue 10, pp 2925–2928 | Cite as

Gomphocarpus mosaic virus, a distinctive member of the genus Potyvirus

  • Ting-Kai Chang
  • Yuh-Kun Chen
Annotated Sequence Record

Abstract

Plants of the species Gomphocarpus physocarpus, commonly known as balloon plant or swan plant, exhibiting virus-like symptoms of mosaic, mottle and crinkling were observed and collected in the southwestern part of Taiwan in 2015. Electron microscopic examination showed the presence of virus-like flexuous-rod particles. Potyvirus pinwheel-shape and laminated inclusion bodies were observed in the preparations of ultrathin sections of diseased leaves. The complete genome sequence of the potyvirus of balloon plant was determined. It is 9998 nucleotides in length, excluding the 3′-terminal poly(A) tail. It contains two open reading frames encoding a polyprotein of 3196 amino acids and a PIPO protein of 89 amino acids. The polyprotein gene shares 52.8-68.4% nucleotide sequence identity and 40.2-75.8% amino acid sequence identity with other potyviruses tested. Molecular analysis indicates that the virus is most closely related to but distinct from keunjorong mosaic virus (KjMV). The virus causing mosaic, mottle and crinkling on Gomphocarpus plants (gomphocarpus mosaic virus, GoMV) likely belongs to a new species of the genus Potyvirus.

Notes

Acknowledgements

We thank Dr. Hei-ti Hsu and Dr. Chung-Chi Hu for critical review of this manuscript.

Funding

This study was funded in part by the Bureau of Animal and Plant Health, Inspection, and Quarantine, Council of Agriculture, Taiwan (Grant no. 106AS-9.5.1-BQ-B4).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

This study did not include experiments with human participants or animals by any of the authors.

Supplementary material

705_2018_3940_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 14 kb)
705_2018_3940_MOESM2_ESM.docx (19 kb)
Supplementary material 2 (DOCX 19 kb)

References

  1. 1.
    Adams MJ, Antoniw JF, Fauquet CM (2005) Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150:459–479CrossRefPubMedGoogle Scholar
  2. 2.
    Adams MJ, Antoniw JF, Beaudoin F (2005) Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol Plant Pathol 6:471–487CrossRefPubMedGoogle Scholar
  3. 3.
    Adams MJ, Zerbini FM, French R, Rabenstein F, Stenger DC, Valkonen JPT (2012) Family Potyviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (ed) Virus taxonomy. In: Ninth report of the International Committee for Taxonomy of Viruses, Elsevier Academic Press, pp 1069–1089Google Scholar
  4. 4.
    Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410CrossRefPubMedGoogle Scholar
  5. 5.
    Bellardi MG, Lisa V (1998) Aggiornamento sulle virosi delle piante ornamentali in Italia. In: Atti Giornate Fitopatologiche 1998. Scicli and Ragusa, Italy, 3–7 May 1998, pp 747–768Google Scholar
  6. 6.
    Bertaccini A, Bellardi MG, Botti S, Paltrinieri S, Restuccia P (2006) Phytoplasma infection in Asclepias physocarpa. Acta Hortic 722:349–354CrossRefGoogle Scholar
  7. 7.
    Chung BY, Miller WA, Atkins JF, Firth AE (2008) An overlapping essential gene in the Potyviridae. Proc Natl Acad Sci USA 105:5897–5902CrossRefPubMedGoogle Scholar
  8. 8.
    Edwardson JR, Christie RG (1991) CRC handbook of viruses infecting legumes. CRC Press, New YorkGoogle Scholar
  9. 9.
    Elliott MS, Massey B, Cui X, Hiebert E, Charudattan R, Waipara N, Hayes L (2009) Supplemental host range of Araujia mosaic virus, a potential biological control agent of moth plant in New Zealand. Aust Plant Pathol 38:603–607CrossRefGoogle Scholar
  10. 10.
    Gal-On A (2000) A point mutation in the FRNK motif of the potyvirus helper component-protease gene alters symptom expression in cucurbits and elicits protection against the severe homologous virus. Phytopathology 90:467–473CrossRefPubMedGoogle Scholar
  11. 11.
    Greber RS (1971) Some characteristics of tobacco streak virus isolated in Queensland. Qld J Agric Anim Sci 28:105Google Scholar
  12. 12.
    Hsu YC, Yeh TJ, Chang YC (2005) A new combination of RT-PCR and reverse dot blot hybridization for rapid detection and identification of potyviruses. J Virol Methods 128:54–60CrossRefPubMedGoogle Scholar
  13. 13.
    Maciel SC, Silva RF, Reis MS, Jadão AS, Rosa DD, Giampan JS, Kitajima EW, Rezende JAM, Camargo LE (2011) Characterization of a new potyvirus causing mosaic and flower variegation in Catharanthus roseus in Brazil. Sci Agric 68:687–690CrossRefGoogle Scholar
  14. 14.
    Miglino R, Druffel KL, Pappu HR (2010) Identification and molecular characterization of a new potyvirus infecting Triteleia species. Arch Virol 155:441–443CrossRefPubMedGoogle Scholar
  15. 15.
    Nam M, Lee JH, Choi HS, Lim HS, Moon JS, Lee SH (2013) Complete genome sequence of keunjorong mosaic virus, a potyvirus from Cynanchum wilfordii. Arch Virol 158:1817–1820CrossRefPubMedGoogle Scholar
  16. 16.
    Puurand U, Makinen K, Paulin L, Saarma M (1994) The nucleotide sequence of potato virus A genomic RNA and its sequences similarities with other potyviruses. J Gen Virol 75:457–461CrossRefPubMedGoogle Scholar
  17. 17.
    Singh M, Singh RP (1996) Nucleotide sequence and genome organization of a Canadian isolate of the common strain of potato virus Y (PVY). Can J Plant Pathol 18:209–224CrossRefGoogle Scholar
  18. 18.
    Turpen T (1989) Molecular cloning of a potato virus Y genome: nucleotide sequence homology in non-coding regions of potyviruses. J Gen Virol 70:1951–1960CrossRefPubMedGoogle Scholar
  19. 19.
    Urcuqui-Inchima S, Haenni AL, Bernadi F (2001) Potyvirus proteins: a wealth of functions. Virus Res 74:157–175CrossRefPubMedGoogle Scholar
  20. 20.
    Weintraub M, Ragetli HW (1970) Distribution of virus like particles in leaf cells of Dianthus barbatus infected with carnation vein mottle virus. Virology 40:868–881CrossRefPubMedGoogle Scholar
  21. 21.
    Wylie SJ, Adams MJ, Chalam C, Kreuze J, López-Moya JJ, Ohshima K, Praveen S, Rabenstein F, Stenger D, Wang A, Zerbini FM (2017) ICTV virus taxonomy profile: Potyviridae. J Gen Virol 98:352–354CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Dl Xu, Liu HY, Kolis ST, Li F, Li R (2010) Biological characterization and complete genomic sequence of Apium virus Y infecting celery. Virus Res 156:917–920Google Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Master Program for Plant Medicine and Good Agricultural PracticeNational Chung Hsing UniversityTaichungTaiwan
  2. 2.Department of Plant PathologyNational Chung Hsing UniversityTaichungTaiwan

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