Archives of Virology

, Volume 163, Issue 10, pp 2805–2810 | Cite as

Whole genome analysis of a novel neurotropic bovine astrovirus detected in a Japanese black steer with non-suppurative encephalomyelitis in Japan

  • Yoshimasa Hirashima
  • Daisuke Okada
  • Shoichi Shibata
  • Shu Yoshida
  • Shoichiro Fujisono
  • Tsutomu Omatsu
  • Tetsuya Mizutani
  • Makoto Nagai
Brief Report


While neurotropic bovine astroviruses (BoAstVs) have been identified in North America and Europe, their presence has never been reported in Asia. In this study, we detected BoAstV in the brain of a steer showing neurological signs. Phylogenetic analysis revealed that the identified virus belongs to the Virginia/Human-Mink-Ovine clade, which contains most of the neurotropic astroviruses including the neurotropic BoAstVs. Similarity plot analysis showed that the virus was closely related to the American BoAstV NeuroS1 strain with respect to the ORF regions and to the European BoAstV CH13 strain in the 3′ untranslated region, suggesting the occurrence of intra-genotypic recombination events.



We thank to Mr. Kazutoshi Matsuzaki for his kind cooperation in the field research. This work was supported by JSPS KAKENHI Grant number 15K07718.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Research involving human participants and/or animals

The present study was carried out according to the Fundamental Guidelines for Proper Conduct of Animal Experiments and Related Activities in Academic Research Institutions under the jurisdiction of the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Supplementary material

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Supplementary material 1 (XLSX 10 kb)
705_2018_3898_MOESM2_ESM.ppt (296 kb)
Supplementary material 2 (PPT 298 kb)
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Supplementary material 3 (PPT 522 kb)


  1. 1.
    De Benedictis P, Schultz-Cherry S, Burnham A, Cattoli G (2011) Astrovirus infections in humans and animals—molecular biology, genetic diversity, and interspecies transmissions. Infect Genet Evol 11:1529–1544CrossRefPubMedGoogle Scholar
  2. 2.
    Donato C, Vijaykrishna D (2017) The broad host range and genetic diversity of mammalian and avian astroviruses. Viruses 9:E102CrossRefPubMedGoogle Scholar
  3. 3.
    Bridger JC, Hall GA, Brown JF (1984) Characterization of a calici-like virus (Newbury agent) found in association with astrovirus in bovine diarrhea. Infect Immun 43:133–138PubMedPubMedCentralGoogle Scholar
  4. 4.
    Cordey S, Brito F, Vu DL, Turin L, Kilowoko M, Kyungu E, Genton B, Zdobnov EM, D’Acremont V, Kaiser L (2016) Astrovirus VA1 identified by next-generation sequencing in a nasopharyngeal specimen of a febrile tanzanian child with acute respiratory disease of unknown etiology. Emerg Microbes Infect 5:e99CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Padmanabhan A, Hause BM (2016) Detection and characterization of a novel genotype of porcine astrovirus 4 from nasal swabs from pigs with acute respiratory disease. Arch Virol 161:2575–2579CrossRefPubMedGoogle Scholar
  6. 6.
    Ng TF, Kondov NO, Deng X, Van Eenennaam A, Neibergs HL, Delwart E (2015) A metagenomics and case-control study to identify viruses associated with bovine respiratory disease. J Virol 89:5340–5349CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Quan PL, Wagner TA, Briese T, Torgerson TR, Hornig M, Tashmukhamedova A, Firth C, Palacios G, Baisre-De-Leon A, Paddock CD, Hutchison SK, Egholm M, Zaki SR, Goldman JE, Ochs HD, Lipkin W (2010) Astrovirus encephalitis in boy with X-linked agammaglobulinemia. Emerg Infect Dis 16:918–925CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Brown JR, Morfopoulou S, Hubb J, Emmett WA, Ip W, Shah D, Brooks T, Paine SM, Anderson G, Virasami A, Tong CY, Clark DA, Plagnol V, Jacques TS, Qasim W, Hubank M, Breuer J (2015) Astrovirus VA1/HMO-C: An increasingly recognized neurotropic pathogen in immunocompromised patients. Clin Infect Dis 60:881–888CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Cordey S, Vu DL, Schibler M, L’Huillier AG, Brito F, Docquier M, Posfay-Barbe KM, Petty TJ, Turin L, Zdobnov EM, Kaiser L (2016) Astrovirus MLB2: A new gastro-enteric virus associated with meningitis and disseminated infection. Emerg Infect Dis 22:846–853CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Blomstrom AL, Widen F, Hammer AS, Belak S, Berg M (2010) Detection of a novel astrovirus in brain tissue of mink suffering from shaking mink syndrome by use of viral metagenomics. J Clin Microbiol 48:4392–4396CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Pfaff F, Schlottau K, Scholes S, Courtenay A, Hoffmann B, Höper D, Beer M (2017) A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep. Transbound Emerg Dis 64:677–682CrossRefPubMedGoogle Scholar
  12. 12.
    Boros Á, Albert M, Pankovics P, Bíró H, Pesavento PA, Phan TG, Delwart E, Reuter G (2017) Outbreaks of neuroinvasive astrovirus associated with encephalomyelitis, weakness, and paralysis among weaned pigs, Hungary. Emerg Infect Dis 23:1982–1993CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Arruda B, Arruda P, Hensch M, Chen Q, Zheng Y, Yang C, Gatto IRH, Ferreyra FM, Gauger P, Schwartz K, Bradner L, Harmon K, Hause B, Li G (2017) Porcine astrovirus type 3 in central nervous system of swine with polioencephalomyelitis. Emerg Infect Dis 23:2097–2100CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Li L, Diab S, McGraw S, Barr B, Traslavina R, Higgins R, Talbot T, Blanchard P, Rimoldi G, Fahsbender E, Page B, Phan TG, Wang C, Deng X, Pesavento P, Delwart E (2013) Divergent astrovirus associated with neurologic disease in cattle. Emerg Infect Dis 19:1385–1392CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Bouzalas IG, Wüthrich D, Walland J, Drögemüller C, Zurbriggen A, Vandevelde M, Oevermann A, Bruggmann R, Seuberlich T (2014) Neurotropic astrovirus in cattle with nonsuppurative encephalitis in Europe. J Clin Microbiol 52:3318–3324CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Seuberlich T, Wüthrich D, Selimovic-Hamza S, Drögemüller C, Oevermann A, Bruggmann R, Bouzalas I (2016) Identification of a second encephalitis-associated astrovirus in cattle. Emerg Microbes Infect 5:e71CrossRefPubMedGoogle Scholar
  17. 17.
    Schlottau K, Schulze C, Bilk S, Hanke D, Höper D, Beer M, Hoffmann B (2016) Detection of a novel bovine astrovirus in a cow with encephalitis. Transbound Emerg Dis 63:253–259CrossRefPubMedGoogle Scholar
  18. 18.
    Selimovic-Hamza S, Bouzalas IG, Vandevelde M, Oevermann A, Seuberlich T (2016) Detection of astrovirus in historical cases of european sporadic bovine encephalitis, Switzerland 1958–1976. Front Vet Sci 3:91CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Bosch A, Pinto RM, Guix S (2014) Human astroviruses. Clin Microbiol Rev 27:1048–1074CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Guix S, Bosch A, Pintó R (2013) Astrovirus taxonomy. In: Schultz-Cherry S (ed) Astrovirus research essential ideas, everyday impacts, future directions. Springer, New York, pp 97–118Google Scholar
  21. 21.
    Woode GN, Bridger JC (1978) Isolation of small viruses resembling astroviruses and caliciviruses from acute enteritis of calves. J Med Microbiol 11:441–452CrossRefPubMedGoogle Scholar
  22. 22.
    Tse H, Chan WM, Tsoi HW, Fan RY, Lau CC, Lau SK, Woo PC, Yuen KY (2011) Rediscovery and genomic characterization of bovine astroviruses. J Gen Virol 92:1888–1898CrossRefPubMedGoogle Scholar
  23. 23.
    Nagai M, Omatsu T, Aoki H, Otomaru K, Uto T, Koizumi M, Minami-Fukuda F, Takai H, Murakami T, Masuda T, Yamasato H, Shiokawa M, Tsuchiaka S, Naoi Y, Sano K, Okazaki S, Katayama Y, Oba M, Furuya T, Shirai J, Mizutani T (2015) Full genome analysis of bovine astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of bovine astrovirus. Arch Virol 160:2491–2501CrossRefPubMedGoogle Scholar
  24. 24.
    Selimovic-Hamza S, Sanchez S, Philibert H, Clark EG, Seuberlich T (2017) Bovine astrovirus infection in feedlot cattle with neurological disease in western Canada. Can Vet J 58:601–603PubMedPubMedCentralGoogle Scholar
  25. 25.
    Spinato MT, Vince A, Cai H, Ojkic D (2017) Identification of bovine astrovirus in cases of bovine non-suppurative encephalitis in eastern Canada. Can Vet J 58:607–609PubMedPubMedCentralGoogle Scholar
  26. 26.
    Mittelholzer C, Hedlund KO, Englund L, Dietz HH, Svensson L (2003) Molecular characterization of a novel astrovirus associated with disease in mink. J Gen Virol 84:3087–3094CrossRefPubMedGoogle Scholar
  27. 27.
    Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882CrossRefPubMedPubMedCentralGoogle Scholar
  28. 28.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedPubMedCentralGoogle Scholar
  29. 29.
    Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS, Novak NG, Ingersoll R, Sheppard HW, Ray SC (1999) Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 73:152–160PubMedPubMedCentralGoogle Scholar
  30. 30.
    Kono R, Hirata M, Kaji M, Goto Y, Ikeda S, Yanase T, Kato T, Tanaka S, Tsutsui T, Imada T, Yamakawa M (2008) Bovine epizootic encephalomyelitis caused by Akabane virus in southern Japan. BMC Vet Res 13(4):20CrossRefGoogle Scholar
  31. 31.
    Katayama T, Saito S, Horiuchi S, Maruta T, Kato T, Yanase T, Yamakawa M, Shirafuji H (2013) Nonsuppurative encephalomyelitis in a calf in Japan and isolation of Japanese encephalitis virus genotype 1 from the affected calf. J Clin Microbiol 51:3448–3453CrossRefPubMedPubMedCentralGoogle Scholar
  32. 32.
    Bouzalas IG, Wüthrich D, Selimovic-Hamza S, Drögemüller C, Bruggmann R, Seuberlich T (2016) Full-genome based molecular characterization of encephalitis-associated bovine astroviruses. Infect Genet Evol 44:162–168CrossRefPubMedGoogle Scholar
  33. 33.
    Selimovic-Hamza S, Boujon CL, Hilbe M, Oevermann A, Seuberlich T (2017) Frequency and pathological phenotype of bovine astrovirus CH13/NeuroS1 infection in neurologically-diseased cattle: towards assessment of causality. Viruses 9:E12CrossRefPubMedGoogle Scholar
  34. 34.
    Deiss R, Selimovic-Hamza S, Seuberlich T, Meylan M (2017) Neurologic clinical signs in cattle with astrovirus-associated encephalitis. J Vet Intern Med 31:1209–1214CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Kagoshima Central Livestock Hygiene Service CenterHiokiJapan
  2. 2.Aira Livestock Hygiene Service CenterKajikiJapan
  3. 3.Nansatsu Livestock Hygiene Service CenterMinamikyushuJapan
  4. 4.Research and Education Center for Prevention of Global Infectious Diseases of AnimalTokyo University of Agriculture and TechnologyFuchuJapan
  5. 5.Department of Bioproduction ScienceIshikawa Prefectural UniversitySuematsuJapan

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