Archives of Virology

, Volume 163, Issue 8, pp 2225–2231 | Cite as

Molecular detection and phylogenetic analysis of megalocytivirus in Brazilian ornamental fish

  • Samara Rita de Lucca MaganhaEmail author
  • Pedro Henrique Magalhães Cardoso
  • Simone de Carvalho Balian
  • Sabrina Ribeiro de Almeida-Queiroz
  • Andrezza Maria Fernandes
  • Ricardo Luiz Moro de Sousa
Brief Report


Megalocytiviruses have a worldwide distribution, causing serious economic loss to the global aquaculture industry. They also present a threat to ornamental fish trade because megalocytiviral infections have unspecified symptoms, making early diagnosis difficult. In this study, 100 ornamental fish from 24 different species were tested by PCR for megalocytivirus, with a 47% positive rate being identified. Phylogenetic reconstruction, based on the major capsid protein (MCP) gene, clustered all Brazilian samples into a single clade, showing identity values ranging from 99% to 100% when compared to each other. This is the first report of megalocytivirus infection in some ornamental fish species in Brazil.



This research was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Grant number 2014/04327-7).


This study was funded by FAPESP (grant number 2014/04327-7).

Compliance with ethical standards

Conflict of interest

Samara Rita de Lucca Maganha declares that she has no conflict of interest. Pedro Henrique Magalhães Cardoso declares that he has no conflict of interest. Simone de Carvalho Balian declares that she has no conflict of interest. Sabrina Ribeiro de Almeida Queiroz declares that she has no conflict of interest. Andrezza Maria Fernandes declares that she has no conflict of interest. Ricardo Luiz Moro de Sousa declares that he has no conflict of interest.

Ethical approval

All applicable institutional guidelines for the care and use of animals were followed (CEUA n° 6782040416).


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Samara Rita de Lucca Maganha
    • 1
    Email author
  • Pedro Henrique Magalhães Cardoso
    • 2
  • Simone de Carvalho Balian
    • 2
  • Sabrina Ribeiro de Almeida-Queiroz
    • 1
  • Andrezza Maria Fernandes
    • 1
  • Ricardo Luiz Moro de Sousa
    • 1
  1. 1.Faculty of Animal Science and Food EngineeringUniversity of Sao PauloPirassunungaBrazil
  2. 2.Faculty of Veterinary Medicine and Animal ScienceUniversity of Sao PauloSão PauloBrazil

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