Archives of Virology

, Volume 163, Issue 7, pp 1985–1988 | Cite as

Characterization and genomic analyses of Aeromonas hydrophila phages AhSzq-1 and AhSzw-1, isolates representing new species within the T5virus genus

  • Shengjian Yuan
  • Ling Chen
  • Quan Liu
  • Yan Zhou
  • Jingfang Yang
  • Deng Deng
  • Hongping Li
  • Yingfei Ma
Annotated Sequence Record


In this study, two bacteriophage isolates, AhSzq-1 and AhSzw-1 that specifically infect Aeromonas hydrophila strain KT998822, were isolated from seawater and characterized. One-step growth curves showed that the latent period of AhSzq-1 and AhSzw-1 are 50 min and 60 min, respectively. The sequence similarities between AhSzq-1 and AhSzw-1 were 88% at the DNA and 83% at the protein level, suggesting that these two phages are representatives of two different species. The virion morphology, DNA genome size and terminal repeats of these two phages are similar to those of viruses classified as T5virus phages. Both phylogenetic analyses and proteomic comparison show that AhSzq-1 and AhSzw-1 group with members of the T5virus genus. We thus propose these two phages as representative isolates of two new species within the T5virus genus.



We are grateful to J. Feng from Institute of Microbiology of the Chinese Academy of Sciences for providing bacterial strains. This study was funded by the Shenzhen Science and Technology Innovation Committee (JCYJ20160229201759414, JCYJ20160122143446357, JSGG20150229145252927, JCYJ20160612152651093, JCYJ20140901003939019, JCYJ20150629151046896 and CXZZ20140901004122088), the Shenzhen Peacock Team Project (KQTD2015033117210153 and KQTD2016112915000294), and the Guangdong Science and Technology Department (2014A020216029). This work was also funded by the National Natural Science Foundation of China (31570115, Y520581001), and National Basic Research Program of China (973 Program: 2014CB745202).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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Supplementary material 5 (DOC 275 kb)


  1. 1.
    Abedon ST, Kuhl SJ, Blasdel BG, Kutter EM (2011) Phage treatment of human infections. Bacteriophage 1:66–85CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Adams MJ, Lefkowitz EJ (2017) Changes to taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2017). Arch Virol 162:2505–2538CrossRefPubMedGoogle Scholar
  3. 3.
    Bailly-Bechet M, Vergassola M, Rocha E (2007) Causes for the intriguing presence of tRNAs in phages. Genome Res 17:1486–1495CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Besemer J, Borodovsky M (2005) GeneMark: web software for gene finding in prokaryotes, eukaryotes and viruses. Nucleic Acids Res 33:W451–W454CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Luo R, Liu B, Xie Y, Li Z, Huang W, Yuan J, He G, Chen Y, Pan Q, Liu Y, Tang J, Wu G, Zhang H, Shi Y, Liu Y, Yu C, Wang B, Lu Y, Han C, Cheung DW, Yiu SM, Peng S, Xiaoqian Z, Liu G, Liao X, Li Y, Yang H, Wang J, Lam TW, Wang J (2012) SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 1:18CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Pajunen M, Kiljunen S, Skurnik M (2000) Bacteriophage phiYeO3-12, specific for Yersinia enterocolitica serotype O:3, is related to coliphages T3 and T7. J Bacteriol 182:5114–5120CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Pedulla ML, Ford ME, Houtz JM, Karthikeyan T, Wadsworth C, Lewis JA, Jacobs-Sera D, Falbo J, Gross J, Pannunzio NR, Brucker W, Kumar V, Kandasamy J, Keenan L, Bardarov S, Kriakov J, Lawrence JG, Jacobs WR Jr, Hendrix RW, Hatfull GF (2003) Origins of highly mosaic mycobacteriophage genomes. Cell 113:171–182CrossRefPubMedGoogle Scholar
  8. 8.
    Radu S, Ahmad N, Ling FH, Reezal A (2003) Prevalence and resistance to antibiotics for Aeromonas species from retail fish in Malaysia. Int J Food Microbiol 81:261–266CrossRefPubMedGoogle Scholar
  9. 9.
    Thompson LR, Zeng Q, Kelly L, Huang KH, Singer AU, Stubbe J, Chisholm SW (2011) Phage auxiliary metabolic genes and the redirection of cyanobacterial host carbon metabolism. Proc Natl Acad Sci USA 108:E757–E764CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Walter W, Sanchez-Cabo F, Ricote M (2015) GOplot: an R package for visually combining expression data with functional analysis. Bioinformatics 31:2912–2914CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Synthetic Biology, Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenChina
  2. 2.Department of Food Science and Engineering, College of Chemistry and Chemical EngineeringShenzhen UniversityShenzhenChina
  3. 3.R&D Center, Shenzhen Alpha Feed Co., LtdShenzhenChina
  4. 4.Medical Institute for Children and Women, Shenzhen Baoan Maternal and Child Health HospitalShenzhenChina

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