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Characterization of a novel RNA virus from the phytopathogenic fungus Leptosphaeria biglobosa related to members of the genus Mitovirus

  • Yong Wang
  • Zhiyong Xu
  • Jiasen Cheng
  • Yanping Fu
  • Daohong Jiang
  • Jiatao XieEmail author
Annotated Sequence Record

Abstract

Mycovirus infection is a universal phenomenon in the major fungus groups. So far, however, mycoviruses have not been described in the phytopathogenic fungus Leptosphaeria biglobosa, the causal agent of phoma stem canker (blackleg). Here, we report the complete genome sequence of a novel mitovirus, Leptosphaeria biglobosa mitovirus 1 (LbMV1), isolated from the strain H3-38 of L. biglobosa. The LbMV1 genome comprises 2568 nucleotides with a low G+C content of 30%. Using the mitochondrial genetic code, the genome of LbMV1 was found to contain a single large open reading frame that encodes a predicted protein of 756 amino acids. Multiple alignment of the predicted protein sequence showed the highest similarity (51% identity) to the putative RNA-dependent RNA polymerase of Ophiostoma mitovirus 4. In addition, phylogenetic analysis grouped LbMV1 within the genus Mitovirus of the family Narnaviridae. Our results suggest that the double-stranded RNA found in strain H3-38 is the replicating genome of the mitovirus LbMV1, its deletion represents the first evidence of a mycovirus infecting L. biglobosa.

Notes

Compliance with ethical standards

Funding

This work was supported by the National Nature Science Foundation of China (31601588), National Key Research and Development Program of China (2017YFD0201100), and the China Agriculture Research System (CARS-13).

Conflict of interest

All authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_4143_MOESM1_ESM.tif (6.1 mb)
Supplementary material 1 (TIFF 6253 kb) Fig. S1 Predicted secondary structures of the 5’ and 3’ termini of the LbMV1/H3-38 genome. (A) The predicted 5’ stem-loop structure (B) The predicted 3’ stem-loop structure
705_2018_4143_MOESM2_ESM.tif (10 mb)
Supplementary material 2 (TIFF 10193 kb) Fig. S2 Alignment of the amino acid sequences of the putative RNA-dependent RNA polymerases of Leptosphaeria biglobosa mitovirus 1 and five other mitoviruses. Six conserved motifs are numbered I to VI
705_2018_4143_MOESM3_ESM.tif (709 kb)
Supplementary material 3 (TIFF 709 kb) Fig. S3 Strain H3-38 was molecularly identified with primers ITS1/ITS4, LmacF/LmacR and LbigF/LmacR. The lanes 1 and 2 represent the DNA amplicons of H3-38 and the negative control (sterile water), respectively. M represents the DNA molecular marker

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Agricultural MicrobiologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  3. 3.Plant Pathology Department, College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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