Complete genome sequence of a novel negevirus isolated from Culex tritaeniorhynchus in China

  • Yuanyuan Wang
  • Xiaofang Guo
  • Honghong Peng
  • Yunlan Lu
  • Xucan Zeng
  • Ke Dai
  • Shuqing Zuo
  • Hongning ZhouEmail author
  • Jiusong ZhangEmail author
  • Yigang TongEmail author
Annotated Sequence Record


A novel negevirus, tentatively named Manglie virus (MaV), was isolated from Culex tritaeniorhynchus from the village of Manglie, Yunnan, China, in August 2011. It was identified by high-throughput sequencing of cell culture supernatants, and the complete genome was sequenced using an Illumina MiSeq sequencer. The complete MaV genome comprised 9,218 nt encoding three hypothetical proteins and had a poly(A) tail. BLASTn analysis showed that the genome had the greatest similarity to Ngewotan virus strain Nepal22, with query coverage of 100% and 79% identity. Genomic and phylogenetic analyses demonstrated that MaV should be considered a novel negevirus.



The authors would like to thank Guangqian Pei, Feiyang Zhao, and Manli Li for their help during this work.


This research was supported by grants from the National Natural Science Foundation of China (No. 81273138 and No. 81572045) and the Yunnan Health Training Project of High-Level Talents (grant numbers D-201604).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2018_4133_MOESM1_ESM.gbk (12 kb)
Supplementary material 1 (gbk 11 kb)
705_2018_4133_MOESM2_ESM.doc (30 kb)
Supplementary material 2 (DOC 29 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Anhui Medical UniversityHefeiChina
  2. 2.State Key Laboratory of Pathogen and BiosecurityBeijing Institute of Microbiology and EpidemiologyBeijingChina
  3. 3.Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and ResearchYunnan Institute of Parasitic DiseasesPu’erChina

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