Establishment and application of an indirect ELISA for porcine circovirus 3
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Porcine circovirus type 3 (PCV3) was initially reported in 2016 in the United States of America. Since then, the virus has been detected on swine farms in Poland, South Korea, and China using PCR. However, a serological survey of PCV3 in pig populations has never been conducted. In this study, for the first time, we established an indirect enzyme-linked immunosorbent (ELISA) assay and performed a national retrospective serological survey for PCV3. Our results showed that the PCV3-postive rate increased from 22.35% to 51.88% between 2015 and 2017. The above results suggest PCV3 has spread widely in China with increased positive rates since 2015.
Compliance with ethical standards
This study was funded by the Major Science and Technology Projects in Henan Province with Grant No.171100110200 and Luoyang Heluo Talent Plan (Dr. Kegong Tian).
Conflict of interest
The authors declare that they do not have any conflict of interest.
All animal trials were approved by the Animal Care and Ethics Committee of China National Research Center for Veterinary Medicine.
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