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Archives of Virology

, Volume 162, Issue 12, pp 3611–3618 | Cite as

Isolation, molecular characterization and an artificial infection model for a variant porcine epidemic diarrhea virus strain from Jiangsu Province, China

  • Hewei Zhang
  • Mingqi Xia
  • Decai Ju
  • Bai Wu
  • Chen Ning
  • Ni Song
  • Teng Feng
  • Feng Chen
  • Xin Wang
  • Ying Wu
  • Wei Wang
  • Shipeng Cheng
  • Wenjie Jin
  • Shucheng Zhang
  • Chunjie Zhang
  • Xiangchao Cheng
  • Ke DingEmail author
  • Hua WuEmail author
Original Article

Abstract

Porcine epidemic diarrhea virus (PEDV) is a causative agent of porcine intestinal disease, which causes vomiting, diarrhea, and dehydration in piglets. PEDV is associated with the most severe pathogenesis in one-week-old piglets, with mortality rates reaching 100%. A PEDV strain was isolated from the intestinal tract of diarrheic piglets from a pig farm in Jiangsu Province in March 2016, termed the JS201603 isolate. The isolated virus was confirmed to be PEDV via RT-PCR, electron microscopy, a cytopathic effect assay and sequence analysis. The S and ORF3 genes of the JS201603 isolate were sequenced, revealing that the S gene was associated with a 15-base insertion at 167 nt, 176 - 186 nt, and 427 - 429 nt, as well as a six-base deletion in 487 - 492 nt, indicating that it was a current epidemic variant compared with the classical strain, CV777. No deletion occurred between 245 - 293 nt of the ORF3 gene in the JS201603 isolate compared with the vaccine isolates YY2013 and SQ2014. An experimental infection model indicated that the piglets in the challenge group successively developed diarrhea, exhibiting yellow-colored loose stools with a foul odor. The piglets in the JS201603 isolate challenge group displayed reduced food consumption, lost weight, and in severe cases even died. No abnormalities were observed in the control group. The JS201603 variant isolated in this study contributes to the evolutionary analysis of diarrhea virus. The experimental infection model has established a foundation for further studies on vaccine development.

Notes

Acknowledgements

This work was supported by the Department of Research and Development of Sinovet (Beijing) Biotechnology Co,.Ltd. The research results belonged to Sinovet (Beijing) Biotechnology Co,.Ltd. Thanks to Wenjie Jin for helping the epidemic survey.

Author contributions

HZ, SC, SZ, NS, CZ, KD, HW designed the study. HZ, MX, DJ, CN, XW, YW, BW, TF, FC, WW performed the experiments. HZ analyzed the data and wrote the manuscript. XC, KD revised the manuscript. WJ helped collecting the samples. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that no competing financial interests exist and no conflicts of interest exist.

Ethical standards

All animal procedures were reviewed and approved by the Institutional Animal Care and Use Committee at Sinovet (Beijing) Biotechnology Co,. Ltd (SYXK:2013-0032).

References

  1. 1.
    Song D, Park B (2012) Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines. Virus Genes 44:167–175CrossRefPubMedGoogle Scholar
  2. 2.
    Pensaert M, De Bouck P (1978) A new coronavirus-like particle associated with diarrhea in swine. Arch Virol 58:243–247CrossRefPubMedGoogle Scholar
  3. 3.
    Flewett T, Boxall E (1976) The hunt for viruses in infections of the alimentary system: an immunoelectron-microscopical approach. Clin Gastroentero l 5:359–385Google Scholar
  4. 4.
    McNulty M, Curran W, McFerran J (1975) Virus-like particles in calves’s faeces. Lancet 306:78–79CrossRefGoogle Scholar
  5. 5.
    Akashi H, Inaba Y, Miura Y, Tokuhisa S, Sato K, Satoda K (1980) Properties of a coronavirus isolated from a cow with epizootic diarrhea. Vet Microbiol 5:265–276CrossRefGoogle Scholar
  6. 6.
    Siddell S, Wege H, Ter Meulen V (1983) The biology of coronaviruses. J Gen Virol 64:761–776CrossRefPubMedGoogle Scholar
  7. 7.
    Bhutani O (1966) Propagation and attenuation of a cylindrical blast wave in magnetogasdynamics. J Math Anal Appl 13:565–576CrossRefGoogle Scholar
  8. 8.
    Xuan H, Xing D, Wang D, Zhu W, Zhao F, Gong H, Fei S (1984) The study on culturing porcine epidemic diarrhea virus with porcine fetal monolayer cell. Chin J Vet Sci 4:202–208Google Scholar
  9. 9.
    Hofmann M, Wyler R (1987) Serologic study of the occurrence of epizootic viral diarrhea in swine in Switzerland. Schweizer Archiv für Tierheilkunde 129:437PubMedGoogle Scholar
  10. 10.
    Li ZL, Zhu L, Ma JY, Zhou QF, Song YH, Sun BL, Chen RA, Xie QM, Bee YZ (2012) Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in south China. Virus Genes 45:181–185CrossRefPubMedGoogle Scholar
  11. 11.
    Kim SY, Goldie SJ, Salomon JA (2009) Cost-effectiveness of Rotavirus vaccination in Vietnam. BMC Public Health 9(1):29CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Chen J, Wang C, Shi H, Qiu H, Liu S, Chen X, Zhang Z, Feng L (2010) Molecular epidemiology of porcine epidemic diarrhea virus in China. Arch Virol Arch Virol 155:1471–1476CrossRefPubMedGoogle Scholar
  13. 13.
    Hao J, Xue C, He L, Wang Y, Cao Y (2014) Bioinformatics insight into the spike glycoprotein gene of field porcine epidemic diarrhea strains during 2011–2013 in Guangdong, China. Virus Genes 49:58–67CrossRefPubMedGoogle Scholar
  14. 14.
    Marthaler D, Jiang Y, Otterson T, Goyal S, Rossow K, Collins J (2012) Complete genome sequence of porcine epidemic diarrhea virus strain USA/Colorado/2013 from the United States. Genome Announc 1(4):e00555–13Google Scholar
  15. 15.
    Reed LJ, Muench H (1938) A simple method of estimating fifty per cent endpoints. Am J Epidemiol 27:493–497CrossRefGoogle Scholar
  16. 16.
    Doane FW, Anderson N (1987) Electron microscopy in diagnostic virology: a practical guide and atlas. Cambridge University Press, CambridgeGoogle Scholar
  17. 17.
    Jung K, Wang Q, Scheuer KA, Lu Z, Zhang Y, Saif LJ (2014) Pathology of US porcine epidemic diarrhea virus strain PC21A in gnotobiotic pigs. Emerg Infect Dis 20(4):662–665CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Huang YW, Dickerman AW, Piñeyro P, Li L, Fang L, Kiehne R, Opriessnig T, Meng XJ (2013) Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States. Mbio 4:00737–00713CrossRefGoogle Scholar
  19. 19.
    Gao Y, Kou Q, Ge X, Zhou L, Guo X, Yang H (2013) Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China. Arch Virol 158:711–715CrossRefPubMedGoogle Scholar
  20. 20.
    Luo Y, Zhang J, Deng X, Ye Y, Liao M, Fan H (2012) Complete genome sequence of a highly prevalent isolate of porcine epidemic diarrhea virus in South China. J Virol 86:9551CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Park SJ, Kim HK, Song DS, An DJ, Park BK (2012) Complete genome sequences of a Korean virulent porcine epidemic diarrhea virus and its attenuated counterpart. J Virol 86:5964CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Chen F, Pan Y, Zhang X, Tian X, Wang D, Zhou Q, Song Y, Bi Y (2012) Complete genome sequence of a variant porcine epidemic diarrhea virus strain isolated in China. J Virol 86:12448CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Lee S, Kim Y, Lee C (2015) Isolation and characterization of a Korean porcine epidemic diarrhea virus strain KNU-141112. Virus Res 208:215–224CrossRefPubMedGoogle Scholar
  24. 24.
    Pan Y, Tian X, Li W, Zhou Q, Wang D, Bi Y, Chen F, Song Y (2012) Isolation and characterization of a variant porcine epidemic diarrhea virus in China. Virol J 9:1CrossRefGoogle Scholar
  25. 25.
    Chen F, Zhu Y, Wu M, Ku X, Ye S, Li Z, Guo X, He Q (2015) Comparative genomic analysis of classical and variant virulent parental/attenuated strains of porcine epidemic diarrhea virus. Viruses 7:5525–5538CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Lee C (2015) Porcine epidemic diarrhea virus: an emerging and re-emerging epizootic swine virus. Virol J 12:193CrossRefPubMedPubMedCentralGoogle Scholar
  27. 27.
    Lee D-K, Park C-K, Kim S-H, Lee C (2010) Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea. Virus Res 149:175–182CrossRefPubMedGoogle Scholar
  28. 28.
    Marthaler D, Jiang Y, Otterson T, Goyal S, Rossow K, Collins J (2013) Complete genome sequence of porcine epidemic diarrhea virus strain USA/Colorado/2013 from the United States. Genome Announc 1:e00555–00513CrossRefGoogle Scholar
  29. 29.
    Masuda T, Murakami S, Takahashi O, Miyazaki A, Ohashi S, Yamasato H, Suzuki T (2015) New porcine epidemic diarrhoea virus variant with a large deletion in the spike gene identified in domestic pigs. Arch Virol 160:2565–2568CrossRefPubMedGoogle Scholar
  30. 30.
    Sato T, Takeyama N, Katsumata A, Tuchiya K, Kodama T, Kusanagi K (2011) Mutations in the spike gene of porcine epidemic diarrhea virus associated with growth adaptation in vitro and attenuation of virulence in vivo. Virus Genes 43:72–78CrossRefPubMedGoogle Scholar
  31. 31.
    Wu M, Chen F, Li Z, Wan S, Ye S, He Q (2016) Isolation, identification and full length genome sequence analysis of a variant porcine epidemic diarrhea virus-CH/YNKM-8/2013. J Huazhong Agric Univ 4:016Google Scholar

Copyright information

© Springer-Verlag GmbH Austria 2017

Authors and Affiliations

  • Hewei Zhang
    • 1
    • 2
    • 3
  • Mingqi Xia
    • 2
  • Decai Ju
    • 2
  • Bai Wu
    • 2
  • Chen Ning
    • 2
  • Ni Song
    • 2
  • Teng Feng
    • 2
  • Feng Chen
    • 2
  • Xin Wang
    • 2
  • Ying Wu
    • 2
  • Wei Wang
    • 2
  • Shipeng Cheng
    • 1
  • Wenjie Jin
    • 2
  • Shucheng Zhang
    • 2
  • Chunjie Zhang
    • 3
  • Xiangchao Cheng
    • 3
  • Ke Ding
    • 3
    Email author
  • Hua Wu
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant SciencesChinese Academy of Agricultural SciencesChangchunChina
  2. 2.Sinovet (Beijing) Biotechnology Co., LtdBeijingChina
  3. 3.The Key Lab of Animal Disease and Public HealthHenan University of Science and TechnologyLuoyangChina

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