Advertisement

Archives of Virology

, Volume 162, Issue 11, pp 3529–3534 | Cite as

Discovery of new orbiviruses and totivirus from Anopheles mosquitoes in Eastern Australia

  • Agathe M. G. Colmant
  • Kayvan Etebari
  • Cameron E. Webb
  • Scott A. Ritchie
  • Cassie C. Jansen
  • Andrew F. van den Hurk
  • Helle Bielefeldt-Ohmann
  • Jody Hobson-Peters
  • Sassan Asgari
  • Roy A. Hall
Brief Report

Abstract

Three new viruses classifiable within the Totivirus and Orbivirus genera were detected from Anopheles mosquito species collected in Eastern Australia. The viruses could not be isolated in C6/36 mosquito cell cultures but were shown to replicate in their mosquito hosts by small RNA analysis. The viruses grouped phylogenetically with other viruses recently detected in insects. These discoveries contribute to a better understanding of commensal viruses in Australian mosquitoes and the evolution of these viruses.

References

  1. 1.
    Hall RA, Bielefeldt-Ohmann H, McLean BJ, O’Brien CA, Colmant AMG, Piyasena TBH, Harrison JJ, Newton ND, Barnard RT, Prow NA, Deerain JM, Mah MGKY, Hobson-Peters J (2016) Commensal viruses of mosquitoes: host restriction, transmission, and interaction with arboviral pathogens. Evol Bioinf Online 12:35–44Google Scholar
  2. 2.
    Hobson-Peters J, Yam AWY, Lu JWF, Setoh YX, May FJ, Kurucz N, Walsh S, Prow NA, Davis SS, Weir R, Melville L, Hunt N, Webb RI, Blitvich BJ, Whelan P, Hall RA (2013) A new insect-specific flavivirus from northern Australia suppresses replication of West Nile virus and Murray Valley encephalitis virus in co-infected mosquito cells. PLoS One 8:e56534CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Hall-Mendelin S, McLean BJ, Bielefeldt-Ohmann H, Hobson-Peters J, Hall RA, van den Hurk AF (2016) The insect-specific Palm Creek virus modulates West Nile virus infection in and transmission by Australian mosquitoes. Parasit Vectors 9:414CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Cook S, Chung BYW, Bass D, Moureau G, Tang S, McAlister E, Culverwell CL, Glücksman E, Wang H, Brown TDK, Gould EA, Harbach RE, de Lamballerie X, Firth AE (2013) Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts. PLoS One 8:e80720CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Shi M, Lin X-D, Tian J-H, Chen L-J, Chen X, Li C-X, Qin X-C, Li J, Cao J-P, Eden J-S, Buchmann J, Wang W, Xu J, Holmes EC, Zhang Y-Z (2016) Redefining the invertebrate RNA virosphere. Nature 540:539–543CrossRefGoogle Scholar
  6. 6.
    Harrison JJ, Warrilow D, McLean BJ, Watterson D, O’Brien CA, Colmant AM, Johansen CA, Barnard RT, Hall-Mendelin S, Davis SS, Hall RA, Hobson-Peters J (2016) A new orbivirus isolated from mosquitoes in North-Western Australia shows antigenic and genetic similarity to Corriparta virus but does not replicate in vertebrate cells. Viruses:8(5). pii: E141. doi: 10.3390/v8050141.Google Scholar
  7. 7.
    Cowled C, Palacios G, Melville L, Weir R, Walsh S, Davis S, Gubala A, Lipkin WI, Briese T, Boyle D (2009) Genetic and epidemiological characterization of Stretch Lagoon orbivirus, a novel orbivirus isolated from Culex and Aedes mosquitoes in northern Australia. J Gen Virol 90:1433–1439CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Attoui H, Mertens P, Becnel J, Belaganahalli S, Bergoin M, Brussaard C (2011) Virus taxonomy: classification and nomenclature. Ninth report of the International Committee on Taxonomy of VirusesGoogle Scholar
  9. 9.
    Roy P, Marshall JJ, French TJ (1990) Structure of the bluetongue virus genome and its encoded proteins. Curr Top Microbiol Immunol 162:43–87PubMedGoogle Scholar
  10. 10.
    Belhouchet M, Mohd Jaafar F, Firth AE, Grimes JM, Mertens PPC, Attoui H (2011) Detection of a fourth orbivirus non-structural protein. PLos One 6:e25697CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Ratinier M, Caporale M, Golder M, Franzoni G, Allan K, Nunes SF, Armezzani A, Bayoumy A, Rixon F, Shaw A, Palmarini M (2011) Identification and characterization of a novel non-structural protein of bluetongue virus. PLos Pathogens 7:e1002477CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Fauver JR, Grubaugh ND, Krajacich BJ, Weger-Lucarelli J, Lakin SM, Fakoli LS 3rd, Bolay FK, Diclaro JW 2nd, Dabire KR, Foy BD, Brackney DE, Ebel GD, Stenglein MD (2016) West African Anopheles gambiae mosquitoes harbor a taxonomically diverse virome including new insect-specific flaviviruses, mononegaviruses, and totiviruses. Virology 498:288–299CrossRefPubMedGoogle Scholar
  13. 13.
    Zhai Y, Attoui H, Mohd Jaafar F, Wang HQ, Cao YX, Fan SP, Sun YX, Liu LD, Mertens PP, Meng WS, Wang D, Liang G (2010) Isolation and full-length sequence analysis of Armigeres subalbatus totivirus, the first totivirus isolate from mosquitoes representing a proposed novel genus (Artivirus) of the family Totiviridae. J Gen Virol 91:2836–2845CrossRefPubMedGoogle Scholar
  14. 14.
    Jansen CC, Prow NA, Webb CE, Hall RA, Pyke AT, Harrower BJ, Pritchard IL, Zborowski P, Ritchie SA, Russell RC, van den Hurk AF (2009) Arboviruses isolated from mosquitoes collected from urban and peri-urban areas of Eastern Australia. J Am Mosq Control Assoc 25:272–278CrossRefPubMedGoogle Scholar
  15. 15.
    Colmant AMG, Hobson-Peters J, Bielefeldt-Ohmann H, van den Hurk AF, Hall-Mendelin S, Chow WK, Johansen CA, Fros J, Simmonds P, Watterson D, Cazier C, Etebari K, Asgari S, Schulz BL, Beebe N, Vet LJ, Piyasena TBH, Nguyen HD, Barnard RT, Hall RA (2017) A New Clade of Insect-Specific Flaviviruses from Australian Anopheles Mosquitoes Displays Species-Specific Host Restriction. mSphere (4):e00262–17. doi: 10.1128/mSphere.00262-17
  16. 16.
    Beebe NW, Ellis JT, Cooper RD, Saul A (1999) DNA sequence analysis of the ribosomal DNA ITS2 region for the Anopheles punctulatus group of mosquitoes. Insect Mol Biol 8:381–390CrossRefPubMedGoogle Scholar
  17. 17.
    Attoui H, Stirling JM, Munderloh UG, Billoir F, Brookes SM, Burroughs JN, de Micco P, Mertens PP, de Lamballerie X (2001) Complete sequence characterization of the genome of the St Croix River virus, a new orbivirus isolated from cells of Ixodes scapularis. J Gen Virol 82:795–804CrossRefPubMedGoogle Scholar
  18. 18.
    Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucl Acids Res:32(5):1792-7Google Scholar
  19. 19.
    Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755CrossRefPubMedGoogle Scholar
  20. 20.
    Colmant AM, Bielefeldt-Ohmann H, Hobson-Peters J, Suen WW, O’Brien CA, van den Hurk AF, Hall RA (2016) A newly discovered flavivirus in the yellow fever virus group displays restricted replication in vertebrates. J Gen Virol. doi: 10.1099/jgv.0.000430 PubMedGoogle Scholar
  21. 21.
    Brackney DE, Scott JC, Sagawa F, Woodward JE, Miller NA, Schilkey FD, Mudge J, Wilusz J, Olson KE, Blair CD, Ebel GD (2010) C6/36 Aedes albopictus cells have a dysfunctional antiviral RNA interference response. PLoS Neglect Trop Dis:4(10):e856. doi: 10.1371/journal.pntd.0000856Google Scholar
  22. 22.
    O’Brien CA, Hobson-Peters J, Yam AWY, Colmant AMG, McLean BJ, Prow NA, Watterson D, Hall-Mendelin S, Warrilow D, Ng M-L, Khromykh AA, Hall RA (2015) Viral RNA intermediates as targets for detection and discovery of novel and emerging mosquito-borne viruses. PLoS Neglect Trop Dis 9:e0003629CrossRefGoogle Scholar
  23. 23.
    van Mierlo JT, van Cleef KW, van Rij RP (2011) Defense and counterdefense in the RNAi-based antiviral immune system in insects. Methods Mol Biol 721:3–22CrossRefPubMedGoogle Scholar
  24. 24.
    Brackney DE, Beane JE, Ebel GD (2009) RNAi targeting of West Nile virus in mosquito midguts promotes virus diversification. PLoS Pathog 5:e1000502CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria 2017

Authors and Affiliations

  • Agathe M. G. Colmant
    • 1
    • 2
  • Kayvan Etebari
    • 2
    • 3
  • Cameron E. Webb
    • 4
    • 5
  • Scott A. Ritchie
    • 6
    • 7
  • Cassie C. Jansen
    • 8
  • Andrew F. van den Hurk
    • 9
  • Helle Bielefeldt-Ohmann
    • 1
    • 2
  • Jody Hobson-Peters
    • 1
    • 2
  • Sassan Asgari
    • 2
    • 3
  • Roy A. Hall
    • 1
    • 2
  1. 1.School of Chemistry and Molecular BiosciencesThe University of QueenslandSt LuciaAustralia
  2. 2.Australian Infectious Diseases Research Centre (AIDRC), The University of QueenslandSt LuciaAustralia
  3. 3.School of Biological SciencesThe University of QueenslandSt LuciaAustralia
  4. 4.Department of Medical Entomology, Centre for Infectious Diseases and Microbiology Laboratory Services, NSW Health PathologyWestmead HospitalWestmeadAustralia
  5. 5.Marie Bashir Institute for Infectious Diseases and BiosecurityUniversity of SydneySydneyAustralia
  6. 6.College of Public Health, Medical and Veterinary SciencesJames Cook UniversityCairnsAustralia
  7. 7.Australian Institute of Tropical Health and MedicineJames Cook UniversityCairnsAustralia
  8. 8.Metro North Public Health UnitMetro North Hospital and Health ServiceWindsorAustralia
  9. 9.Public Health Virology, Forensic and Scientific Services, Department of HealthQueensland GovernmentCoopers PlainsAustralia

Personalised recommendations