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Archives of Virology

, Volume 162, Issue 11, pp 3549–3550 | Cite as

Complete genomic sequence of a duck enteritis virus attenuated via serial passage in chick embryos

  • Chenghuai YangEmail author
  • Bing Zhang
  • Junping Li
  • Xiaojie Huang
  • Dan Liu
  • Lidan Hou
  • Qihong Li
  • Huijiao Li
Annotated Sequence Record

Abstract

Here, we present the complete genomic sequence of an attenuated duck enteritis virus (DEV). The Chinese standard challenge strain of DEV (DEV CSC) was serially passaged 20 times in chick embryo fibroblasts and then 85 times in chick embryos. The virus was attenuated and was avirulent to 2-month-old ducks. The attenuated DEV genome is 162,131 base pairs (bp) in length and as long as the parental genomic sequence. There are only 22 nucleotide substitutions, resulting in single amino acid changes in open reading frames LORF5, LORF4, UL41, UL39, UL32, UL13, UL10, UL3, US3, US4 and US7. The genome sequence has been deposited in the GenBank database under accession number KU216226. This study provides genetic information about DEV attenuation and further advances our understanding of the molecular basis of DEV pathogenesis.

Notes

Acknowledgements

This work was supported by the National Key Research and Development Program of China (No. 2017YFD0500800) and Beijing Natural Science Foundation (6162025).

Compliance with ethical standards

Funding

This work was supported by the National Key Research and Development Program of China (No. 2017YFD0500800) and Beijing Natural Science Foundation (6162025).

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

The animal experimental protocols were approved by the Animal Care and Protection Committee, China Institute of Veterinary Drug Control. All animal experiments were carried out in accordance with the requirements of the Regulations of Experimental Animal Administration of the People’s Republic of China. All efforts were made to minimize suffering.

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Copyright information

© Springer-Verlag GmbH Austria 2017

Authors and Affiliations

  1. 1.China Institute of Veterinary Drug ControlBeijingPeople’s Republic of China

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